The rapid spread of animal diseases and pathogen evolution have long posed significant threats to the healthy development of animal husbandry. Traditional nucleic acid detection methods such as PCR rely on expensive equipment and professional personnel, making them difficult to meet the needs of on-site scenarios such as farms and rural areas.

Methane is one of the key greenhouse gases affecting global climate. Although its atmospheric lifetime is shorter than that of carbon dioxide, reducing methane emissions in the short term is more effective in slowing global warming.