In the January 8 Proceedings of the National Academy of Sciences, researchers from the Smithsonian Tropical Research Institute (STRI), The Cucurbit Network and the University of Puerto Rico establish mitochondrial DNA analysis as a powerful tool for understanding relationships among flowering plants. A comparison of mtDNA from cultivated squash, pumpkins, gourds and their wild ancestors strongly supports hypotheses based on archeological and ethnobotanical evidence for six independent domestication events in the New World.