Gasdermin D knockout in alcohol-induced ACLF increases kidney damage (IMAGE)
Caption
(A) Kidney histopathological score assessing tubular dilation, interstitial hypercellularity, cortical and medullary tubular casts and neutrophil infiltration shown in the representative images from H&E staining in kidney sections (20× and 40× magnification). (B) BUN serum levels. (C) KIM-1 expression in the serum was assessed by ELISA. (D) Renal mRNA expression of neutrophil infiltration markers including Ly6g and Cxcl1. (E) Renal mRNA expression of senescence and cell death markers including Cdkn2a, Cdkn1a, Serpin1, proCasp3, Fas, Tnfrsf10b, Bcl2, Ripk1, Ripk3, Mlkl, Nlrp3, Asc, proCasp1, Gsdmd and Il-18. qPCR levels are normalised to WT ACLF group, and the genes that significantly change are highlighted in bold. Scale bar for 200×: 50 µm; for 400×: 20 µm. Data are expressed as mean±SD (n=6–9 per group). Statistical significance was determined using two-tailed t-test: *p<0.05, **p<0.005. ACLF, acute-on-chronic liver failure; BUN, blood urea nitrogen; Cxcl1, C-X-C motif chemokine ligand 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GSDM-D, gasdermin D; KIM-1, kidney injury marker 1; KO, knockout; mRNA, messenger RNA; qPCR, quantitative PCR; WT-wild-type.
Credit
By Martí Ortega-Ribera, Yuan Zhuang, Veronika Brezani, et al.
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