Gasdermin D deficient mice undergoing ACLF exhibit reduced levels of circulating monocytes and decreased hepatic inflammation (IMAGE)

First Hospital of Jilin University

Gasdermin D deficient mice undergoing ACLF exhibit reduced levels of circulating monocytes and decreased hepatic inflammation

Caption

(A) Schematics of the murine model of ACLF in WT or GSDM-D KO mice. (B) Frequency of circulating monocytes (Ly6Chigh and Ly6Clow cells) was analysed by flow cytometry. (C) Hepatic mRNA expression of inflammation markers including Mcp1, Ccr2, Cd206, Cd32 and Tnfa. (D) Hepatic protein expression of inflammatory markers including IL-6 and MCP1 assessed by ELISA. (E) Representative images and quantification (% positive area) of CD68 immunohistochemistry in the liver. Scale bar for 200×: 50 µm. Data are expressed as mean±SD (n=6–9 per group). Statistical significance was determined using two-tailed t-test: *p<0.05, **p<0.001, ***p<0.0001. ACLF, acute-on-chronic liver failure; BDL, bile duct ligation; EtOH, alcohol; GSDM-D, gasdermin D; IL-6, interleukin 6; KO, knockout; MCP1, monocyte chemoattractant protein 1; mRNA, messenger RNA; WT, wild-type.

Credit

Martí Ortega-Ribera, Yuan Zhuang, Veronika Brezani, et al.

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