image: Twenty-four hour composite samples are collected from a building sewer line, targeted subregion, or influent to a wastewater treatment plant (denoted by asterisks). Replicate subsamples of the composite samples are spiked with bovine coronavirus and RNA is extracted (4S method). RT-qPCR assays are performed in triplicate to quantify SARS-CoV-2 (CDC-N1 target), pepper mild mottle virus (PMMoV), and bovine coronavirus (BCoV). Data analysis steps include combining RT-qPCR technical triplicates, checking standards and negative controls, and calculating gene copies per milliliter of raw wastewater. view more
Credit: Kantor et al., 2022, PLOS Water, CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/)
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Article URL: https://journals.plos.org/water/article?id=10.1371/journal.pwat.0000007
Article Title: Operationalizing a routine wastewater monitoring laboratory for SARS-CoV-2
Author Countries: U.S.A.
Funding: Funding was received from the Catena Foundation, the Center for Information Technology Research in Service to Society, and the Innovative Genomics Institute to KLN. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Article Title
Operationalizing a routine wastewater monitoring laboratory for SARS-CoV-2
Article Publication Date
15-Feb-2022
COI Statement
RSK is a friend of the former Executive Editor of PLOS Water. The remaining authors have declared that no competing interests exist.