The workflow for sampling, testing, and data analysis (IMAGE)
Caption
Twenty-four hour composite samples are collected from a building sewer line, targeted subregion, or influent to a wastewater treatment plant (denoted by asterisks). Replicate subsamples of the composite samples are spiked with bovine coronavirus and RNA is extracted (4S method). RT-qPCR assays are performed in triplicate to quantify SARS-CoV-2 (CDC-N1 target), pepper mild mottle virus (PMMoV), and bovine coronavirus (BCoV). Data analysis steps include combining RT-qPCR technical triplicates, checking standards and negative controls, and calculating gene copies per milliliter of raw wastewater.
Credit
Kantor et al., 2022, PLOS Water, CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/)
Usage Restrictions
Credit must be given to the creator.
License
CC BY