image: Figure 1. Ce6-GFFY prohibits colorectal cancer growth and has little side effects. Agents were injected through the tail vein of the CT26-derived subcutaneous tumor mice model, and the 660 nm, 0.2 W/cm2 laser irradiation (1 min on, 1 min off; 4 cycles) was performed 6 h after the injection. Only a single dose was administered during the entire treatment cycle. (A) Schematic diagram of the PDT strategy. PDT, photodynamic therapy. (B–D) Mice were treated with PBS, GFFY (2.5 mg/kg), Ce6 (2.5 mg/kg), or Ce6-GFFY (5 mg/kg), and tumor tissues were collected (B) and weighed (C) after treatment, and tumor growth curve was analyzed during treatment (D). n = 4. (E) Mice body weight was analyzed during treatment. n = 4. (F) Pathological analysis of hearts, livers, spleens, lungs, and kidneys derived from the indicated agents treated mice using hematoxylin-eosin (H&E) staining. n = 4. “L” in “PBS + L”, “GFFY + L”, “Ce6+L”, “Ce6-GFFY + L”: laser irradiation. Scale bar, 200 μm. Statistical analyses were performed using two-way ANOVA; bars, standard deviation; n.s., not significant; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001.
Credit: Genes & Diseases
A new publication from Genes & Diseases; DOI 10.1016/j.gendis.2024.101441, discusses how Ce6-GFFY is a novel photosensitizer for colorectal cancer therapy.
Photodynamic therapy is an “old” strategy for cancer therapy featuring clinical safety and rapid working, but suitable photosensitizers for colorectal cancer therapy remain lacking.
This study synthesized a novel photosensitizer termed Ce6-GFFY based on a self-assembling peptide GFFY and a photo-responsive molecule chlorin e6 (Ce6). Ce6-GFFY forms macroparticles with a diameter of ∼160 nm and possesses a half-life of 10 h, as well as an ideal tumor-targeting ability in mouse models. Ce6-GFFY effectively penetrates cells and generates numerous reactive oxygen species upon 660 nm laser irradiation. The reactive oxygen species promotes the accumulation of cytotoxic T cells and decrease of myeloid-derived suppressor cells in the tumor microenvironment through immunogenic cell death, thus prohibiting the growth of both primary and metastatic tumors after once treatment.
This research not only provides a strategy for photosensitizer development but also confirms a promising application of Ce6-GFFY for colorectal cancer therapy.
Keywords: Anti-tumor immunity, Ce6-GFFY, Colorectal cancer, Novel photosensitizer, Photodynamic therapy
# # # # # #
Genes & Diseases publishes rigorously peer-reviewed and high quality original articles and authoritative reviews that focus on the molecular bases of human diseases. Emphasis is placed on hypothesis-driven, mechanistic studies relevant to pathogenesis and/or experimental therapeutics of human diseases. The journal has worldwide authorship, and a broad scope in basic and translational biomedical research of molecular biology, molecular genetics, and cell biology, including but not limited to cell proliferation and apoptosis, signal transduction, stem cell biology, developmental biology, gene regulation and epigenetics, cancer biology, immunity and infection, neuroscience, disease-specific animal models, gene and cell-based therapies, and regenerative medicine.
Scopus CiteScore: 7.3
Impact Factor: 6.9
# # # # # #
More information: https://www.keaipublishing.com/en/journals/genes-and-diseases/
Editorial Board: https://www.keaipublishing.com/en/journals/genes-and-diseases/editorial-board/
All issues and articles in press are available online in ScienceDirect (https://www.sciencedirect.com/journal/genes-and-diseases ).
Submissions to Genes & Disease may be made using Editorial Manager (https://www.editorialmanager.com/gendis/default.aspx ).
Print ISSN: 2352-4820
eISSN: 2352-3042
CN: 50-1221/R
Contact Us: editor@genesndiseases.com
X (formerly Twitter): @GenesNDiseases (https://x.com/GenesNDiseases )
# # # # # #
Wei Qiao, Shuxin Li, Linna Luo, Meiling Chen, Xiaobin Zheng, Jiacong Ye, Zhaohui Liang, Qiaoli Wang, Ting Hu, Ling Zhou, Jing Wang, Xiaosong Ge, Guokai Feng, Fang Hu, Rongbin Liu, Jianjun Li, Jie Yang, Ce6-GFFY is a novel photosensitizer for colorectal cancer therapy, Genes & Diseases, Volume 12, Issue 2, 2025, 101441, ISSN 2352-3042, https://doi.org/10.1016/j.gendis.2024.101441
Journal
Genes & Diseases