video: A representative cluster of cells with microtubule (MT) protrusions comprises the early interplanar amida network (IPAN). The inset on the left provides an apical view of a subset of the cells shown in a lateral view on the right. Cells with MT protrusions are connected to each other via lateral actin-based filopodia and undergo bundling as some of their MTs disassemble.
The MT subunits generated from disassembly of protrusion MTs are thought to contribute to the formation of the mitotic spindle within the dividing cell
Credit: The animation was created by Erich Brutus.
Live imaging unveils key cell dynamics for 3D organ formation in Drosophila.
Animal development requires successive changes in cell and tissue structures. To form complex 3D organs, cell shapes must adapt to support tissue morphogenesis. However, our understanding of how cellular structure changes are coupled with dynamic tissue morphogenesis is limited, largely due to reliance on studies of fixed tissues and cultured cells. Real-time observation of cell shape changes during morphogenesis is therefore crucial.
Researchers from the University of Tartu, Estonia, and the University of Helsinki, Finland, have introduced a 5D in vivo live imaging protocol to observe 3D tissue dynamics with high resolution. They discovered that dorsal and ventral cells in the fruit fly Drosophila melanogaster’s pupal wing form a cellular network, the Interplanar Amida Network (IPAN), through basal microtubule (MT) protrusions. This network sustains cellular connections during early inflation stages and supports 3D tissue growth by allowing MTs to reorganize into mitotic spindles following programmed disassembly of cell-cell contacts.
This study not only reveals the physiological significance of the IPAN but also provides insights into the challenges of live-imaging and genetic manipulation of protrusions. The findings suggest that the loss of cell-cell contacts functions as a key regulator of coordinated mitoses, a mechanism that may be applicable to 3D morphogenesis in multicellular organisms. The use of powerful Drosophila genetics tools in combination with multi-colored fluorescent in vivo live-imaging offers a comprehensive system to address questions about cell shape changes impacting tissue morphogenesis.
Including movies in the article will effectively present the data, highlighting the unique cellular mechanisms of 3D organ formation of broad biological interest.
Journal
The EMBO Journal
Method of Research
Experimental study
Subject of Research
Animals
Article Title
Programmed disassembly of a microtubule-based membrane protrusion network coordinates 3D epithelial morphogenesis in Drosophila
Article Publication Date
23-Jan-2024
COI Statement
N/A