Figure 1. (IMAGE)

Osaka University

Figure 1.

Caption

<b>EGF prohibits the differentiation of cells within a hiPSC-derived SEAM into a corneal epithelial phenotype.</b> (A) iPSCs differentiate into four concentric zones in a SEAM and cells in zone 3 have characteristics of the ocular surface ectoderm. (B) Immunostaining of the EGF-treated SEAM (+EGF) and the KGF-treated SEAM (+KGF). In the EGF-treated SEAM, K12 (a corneal epithelial cell marker) was not detected at 10 weeks after the start of differentiation. (C) Gene expression analysis revealed that corneal epithelial cell markers (K12 and K3) expressions were downregulated significantly (**<i>p</i> < 0.01), which similar to the results of immunostaining.

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Osaka University

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