Figure 2. (IMAGE)

Osaka University

Figure 2.

Caption

<b>KGF-medium maturation culture of the P2 fraction cells obtained from EGF-treated SEAMs can reconstitute a functional conjunctival epithelium that includes goblet cells.</b> (A) FACS analysis of CD200, SSEA-4, and ITGB4 for the EGF-treated SEAM derivative after 10-14 weeks of culture. (B) Gene expression analysis of conjunctival epithelium-related markers for the P2 and P3 cells before and after maturation culture. (C) Immunostaining for MUC5AC of the P2 and P3 fraction cells after maturation culture. (D) The density of MUC5AC-positive cells. (E) ELISA for MUC5AC in culture supernatants. (F) Immunostaining of human iPS cell-derived conjunctival epithelium. (G) PAS staining.

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Osaka University

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