Fig.1 Schematic Diagram of Cell Transfer Technology and Double-Cell Transfer (IMAGE)
Caption
(A) We coated the surface of glass substrate with tetraethylene glycol (TEG, brown) and the layer was partially degraded by UV irradiation to prepare hydrophilic cell adhesive surface (green). Cells to be transferred were poured onto the substrate and incubated to allow the cells to adhere to the substrate surface. Transfer substrate with cells was then placed onto the scaffold (amnion) in the direction of cell surface down. Cells were further cultured and transfer substrate was carefully removed subsequently. Cells are transferred onto scaffold surface. (B) Cells of the first layer (green) were seeded on the transfer substrate and cultured. Then, the cells of the second layer (red) were seeded onto the cells of the first layer. After incubation, transfer substrate bearing two layers of cells was placed onto the amnion to make direct contact between cells and scaffold surface. Double cell layers were transferred onto the scaffold material after the removal of the transfer base.
Credit
Department of Nanomedicine (DNP), TMDU
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