Development of Cal-Light System (IMAGE)
Caption
Schematic drawing of Cal-Light system. M13 and calmodulin proteins are fused to c-terminus and n-terminus of TEV protease (TEV-C and TEV-N), respectively. When Ca2+ arises in the cytosol, M13 and calmodulin bind to each other and subsequently TEV-C and TEV-N regain proteolytic functions. However, TEV protease cannot recognize TEVseq easily in a dark condition, because TEVseq is inserted at the c-terminus of AsLOV2 J-helix. Blue light causes a conformational change of J-helix making TEVseq unmasked. Cleaved tTA translocates to the nucleus and initiates gene expression.
Credit
Max Planck Florida Institute for Neuroscience
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