Labeling Active Population of Neurons during Complex Behavior (IMAGE)
Caption
(TOP) To label learning-related neuronal population, we trained water restricted mice to learn repetitive lever pressing behavior to get water rewards. Fiber optics were implanted both hemispheres of M1 area and blue laser was programmed to be switched on for 5 sec whenever mice press the lever. Once light was on for 5 seconds, the next blue light was prohibited for the following 25 sec even though mice press the lever. (BOTTOM) Representative images from mouse brains trained with or without blue light; cumulative distribution of G/R at each condition (Light only: n = 448 cells / 8 mice; Activity only: n = 585 cells / 9 mice; Light + Activity: n = 504 cells / 11 mice); and summary box plot chart of G/R (Light only: 0.32 ± 0.09, n = 448; Activity only: 0.36 ± 0.11, n = 585, p = 0.34; Light + Activity: 1.1 ± 0.97, n = 504, p < 0.005).
Credit
Max Planck Florida Institute for Neuroscience
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