Figure 1 (IMAGE)
Caption
Above: confocal fluorescence microscope image of yeast cells: Image shows the enhanced green fluorescent protein (eGFP) (in green) and the vacuolar membranes (in red) in the cells. The Sed1-anchored eGFPs (left) are mainly located on the cell surface, whereas a part of the Sag1-anchored eGFPs are located in the intracellular vacuoles and the amount of Sag1-anchored eGFP transported to the cell surface is comparatively low.
Below: Immunoelectron-microscope image showing the yeast cell wall: The arrowheads indicate the location of the eGFP. Sed1-anchored eGFP is mostly located outside the cell wall, whereas Sag1-anchored eGFP is mostly positioned inside the cell wall.
Credit
Kobe University
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