Figure 2 (IMAGE)

_Tokyo Medical and Dental University

Figure 2

Caption

A. Live-cell fluorescence polarization imaging of the first cleavage of a starfish embryo expressing POLArIS that specifically binds to F-actin. Vertical and horizontal actin filaments are shown in magenta and green, respectively. FLARE structures made of radially extending actin filaments were observed as cross-patterns of magenta and green. Scale bar, 50μm. B. A starfish embryo in the first cleavage was fixed, stained with fluorescent-dye-conjugated phalloidin (stains F-actin) and anti-tubulin antibody (stains microtubules), and observed with confocal laser scanning microscope. Yellow circles, regions of FLARE structure; Red circles, regions of astral microtubules; green box, mitotic spindle; orange arrows, centrosomes. Scale bar, 100 μm.

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Department of Neuroanatomy and Cellular Neurobiology, TMDU

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