Experimental Procedure for Gene Knockout using the Pkir Vector (IMAGE)

Institute of Transformative Bio-Molecules (ITbM), Nagoya University

Experimental Procedure for Gene Knockout using the Pkir Vector

Caption

Plant seeds containing Cas9 are marked with a red fluorescent marker to tell which seeds contain Cas9. Using this method, it becomes possible to obtain seeds that have a knockout mutation in their target gene and do not contain Cas9, in the second generation by selection. This whole process takes about 3 months.

Credit

ITbM, Nagoya University

Usage Restrictions

None

License

Licensed content

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.