Experimental Procedure for Gene Knockout Using the pKIR Vector (IMAGE)

Institute of Transformative Bio-Molecules (ITbM), Nagoya University

Experimental Procedure for Gene Knockout Using the pKIR Vector

Caption

Plant seeds containing Cas9 are marked with a red fluorescent marker to tell which seeds contain Cas9. Using this method, it becomes possible to obtain seeds that have a knockout mutation in their target gene and do not contain Cas9, in the second generation by selection. This whole process takes about three months.

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ITbM, Nagoya University

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