Cartoon Illustration (IMAGE)
Caption
(A) Cartoon illustration depicting where the flox sites are engineered within the Uhrf1 gene locus. (B) Cartoon illustration depicting where the stop codon is engineered with respect to known domains in the UHRF1 protein in Uhrf1hepKO mice. (C) PCR amplification of the Cre transgene gDNA isolated from various tissues of Uhrf1hepKO mice (primer sequences found in supplementary table) with the 422 bp amplicon indicates presence of the Cre transgene and the 3957 bp amplicon corresponding to the wild-type allele. (D) PCR amplification of Uhrf1 floxed or the corresponding WT alleles from gDNA isolated from whole liver tissue of homozygous floxed (Uhrf1fl/fl), heterozygous floxed (Uhrf1fl/+), or WT (Uhrf1+/+) mice. (E) UHRF1 protein detected by immunofluorescence in WT or Uhrf1hepKO mouse liver cryosections at 36 hours post-PH (N=1). (F) Expression of Uhrf1 in the liver of WT or Uhrf1hepKO mouse at 40 hours post-PH (time-point of maximum Uhrf1 detection in regenerating liver of WT mice) detected by RNAseq as displayed on UCSC genome browser (N=1). Error bars represent s.d.
Credit
Kirsten Sadler Edepli, NYU Abu Dhabi Associate Professor of Biology
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