Effect of miR-146b overexpression on islet beta-cell apoptosis. (IMAGE)
Caption
(A) Min6 cells were transfected with 25 nM miR-146b mimics and incubated with annexin-V and propidium iodide for 72 h. The apoptosis of cells was detected with a flow cytometer. The data were presented as the apoptosis rate (apoptotic cell numbers/total cell numbers). (B, C) Primary mouse islets were transfected with 25 nM miR-146b mimics and incubated with annexin-V or TUNEL for 72 h. The data were presented as a ratio (annexin-V/TUNEL fluorescence intensity/nuclei fluorescence intensity per islet area). (D) Primary human islets were transfected with 25 nM miR-146b mimics and incubated with annexin-V for 72 h. The data were presented as a ratio (annexin-V fluorescence intensity/nuclei fluorescence intensity per islet area). (E) The abundance of apoptotic proteins in transfected Min6 cells was measured by western blotting. Representative blots are shown on the top. (F) The gene expression of Bcl family members in transfected Min6 cells was quantified by real-time quantitative PCR. In all the experiments, the cells or islets transfected with the miR-mimic negative control were taken as the controls (mimics NC). Results are mean ± standard error of the mean for three experiments. *P < 0.05 and ***P < 0.001 versus mimics NC.
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Genes & Diseases
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