The exosomal miRNA expression in the circulation of the subjects, the tissue source of circulating up-regulated miR-146b, and the direct effects of high glucose, free fatty acids (FFAs), and inflammatory cytokines on islet miR-146b expression. (IMAGE)
Caption
(A) The seven miRNAs, which were significantly up-regulated (fold-change > 2) and are known to be functional in cell survival, were further quantified by real-time quantitative PCR. Expression levels of these miRNAs were compared among three groups of subjects (n = 20 subjects/group). The values were presented as relative expression compared with healthy weight. Results are mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, and ***P < 0.001 versus healthy weight. +P < 0.05 versus obesity. (B, C) Expression of miR-146b and miR-134 was quantified by real-time quantitative PCR in plasma exosomes, cultured tissue explants, and culture medium exosomes of db/db obese diabetic mice. The values were presented as relative expression compared with their wild-type littermates (LMs). Results are mean ± SEM for 8–10 mice/group. *P < 0.05, **P < 0.01, and ***P < 0.001 versus respective LMs. (D) Min6 cells, primary mouse islets, and primary human islets were cultured with or without high glucose, FFAs, or inflammatory cytokines for 24 h. Expression of miR-146b was quantified by real-time quantitative PCR. The values were presented as relative expression compared with the control (5 mM glucose). Results are mean ± SEM for three experiments. *P < 0.05 and **P < 0.01 versus control.
Credit
Genes & Diseases
Usage Restrictions
Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.
License
CC BY-NC-ND