HMGN2 promotes glioblastoma multiforme proliferation through CDC20 regulating cell cycle. (IMAGE)
Caption
(A) The levels of cell cycle-related proteins were examined by Western blot in LN229 and U-87 MG cells of the NC, scramble, and HMGN2 knockdown groups. (B) The mRNA levels of cell cycle-related genes were examined by qPCR in LN229 and U-87 MG cells of the NC, scramble, and HMGN2 knockdown groups. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001. (C) CDC20 and CCNB1 expression levels were detected by Western blot in the CDC20 rescue assay. (D) The proliferation ability of the CDC20 rescue assay in LN229 cells was evaluated by CCK-8 assay. (E) Cell cycle distribution in the CDC20 rescue assay was detected by flow cytometry. The mean percentages of the population in each phase are shown on the right. All data were expressed as mean ± standard deviation. Student's t-test was performed to analyzed significance; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, and ∗∗∗∗P < 0.0001.
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Genes & Diseases
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