IARS2 affected WNT signaling via inhibiting degradation of β-catenin. (IMAGE)
Caption
(A) Relative CTNNB1 mRNA level in normal and pancreatic adenocarcinoma cell lines. (B) The correlation between relative IARS2 mRNA transcription level and CTNNB1 mRNA transcription level. (C) CTNNB1 mRNA level remained unchanged after IARS2 knockdown or overexpression. (D) Downstream targets of CTNNB1 were up-regulated in IARS2 overexpressed cell line and down-regulated in IARS2 knocked down cell line. (E) Cycloheximide assays showed IARS2 regulated the protein half-life of β-catenin. (F) IARS2 knockdown increased β-catenin phosphorylation at Ser33/37 site while overexpression reduced phosphorylation. (G) Proteasome inhibitor MG-132 (10 μM) restored β-catenin expression in IARS2 silenced PANC-1 cells. Grey value of brands was measured by ImageJ and data represented the mean ± standard deviation of at least three experiments. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. IARS2, isoleucyl-tRNA synthetase 2; CTNNB1, catenin beta 1.
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Genes & Diseases
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