The effects of Shh on BMP9-induced early osteogenic differentiation of MSCs. (IMAGE)

Compuscript Ltd

The effects of Shh on BMP9-induced early osteogenic differentiation of MSCs.

Caption

(A) The infection of Ad-Shh in MSCs (fluorescence microscope, × 100). (B) Validation of the expression of Shh by Ad-Shh in MSCs (Western blot). (C, D) The effects of Shh on BMP9-induced ALP activity in C3H10T1/2 cells (C: chemiluminescence; D: cytochemical staining). (E, F) The effects of Shh on BMP9-induced ALP activity in C2C12 cells (E: chemiluminescence; F: cytochemical staining). (G, H) The effects of Shh on BMP9-induced ALP activity in MEFs (G: chemiluminescence; H: cytochemical staining). ∗p < 0.05, ∗∗p < 0.01; ALP, alkaline phosphatase; BMP9, bone morphogenetic protein 9; MSCs, mesenchymal stem cells; Shh, Sonic Hedgehog.

Credit

Genes & Diseases

Usage Restrictions

Credit must be given to the creator. Only noncommercial uses of the work are permitted. No derivatives or adaptations of the work are permitted.

License

CC BY-NC-ND

Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.