The effects of Gli1/Gli2 inhibitor GANT-61 on the enhancing impacts of Shh on BMP9-induced osteogenic differentiation of MSCs. (IMAGE)

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The effects of Gli1/Gli2 inhibitor GANT-61 on the enhancing impacts of Shh on BMP9-induced osteogenic differentiation of MSCs.

Caption

(A) The effects of GANT-61 on the enhancing effects of Shh on BMP9-induced ALP activity in C3H10T1/2 cells (chemiluminescence). (B) The effects of GANT-61 on the enhancing effects of Shh on BMP9-induced ALP activity in C3H10T1/2 cells (cytochemical staining). (C) The effects of GANT-61 on the enhancing effects of Shh on BMP9-induced matrix mineralization in C3H10T1/2 cells (alizarin red S staining). (D) The effects of GANT-61 on the enhancing effects of Shh on BMP9-induced ALP activity in C2C12 cells (cytochemical staining). (E) The effects of GANT-61 on the enhancing effects of Shh on BMP9-induced matrix mineralization in C2C12 cells (alizarin red S staining). (F) The effects of GANT-61 on the enhancing effects of Shh on BMP9-induced Samd1/5/8 transcriptional activity in C3H10T1/2 cells (luciferase reporter assay). ∗p < 0.05, ∗∗p < 0.01. ALP, alkaline phosphatase; BMP9, bone morphogenetic protein 9; Gli1/2, glioma-associated oncogene 1/2; MSCs, mesenchymal stem cells; Shh, Sonic Hedgehog.

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Genes & Diseases

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