Experimental outline (IMAGE) Stockholm University Caption Figure 1A in the study. Schematic outline, illustrating the experimental approaches used to characterize transcriptional changes during P. falciparum male and female gametocyte development, using the P. falciparum gametocyte-producing NF54 peg4-tdTomato transgenic cell line. The schematic highlights FACS-based parasite population enrichment followed by 10X 3’ single-cell RNA-seq (10X Genomics) and highlights two of the computational analyses in the study. Panel A was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en). Credit Stockholm University/Nat Comm graphical abstract/CC BY 4.0 Usage Restrictions May be used in connection to the related research. License Original content Disclaimer: AAAS and EurekAlert! are not responsible for the accuracy of news releases posted to EurekAlert! by contributing institutions or for the use of any information through the EurekAlert system.