Figure 3 (IMAGE)
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Summary of the mechanism of trabectedin-induced TC-NER-mediated break formation and break mapping by TRABI-Seq. Trabectedin-DNA adducts are recognized by TC-NER and lead to an abortive reaction as these adducts block the incision of the XPG endonuclease, causing persistent XPF-mediated breaks. TRABI-Seq (TRABectidin-Induced break sequencing) was used to map those breaks in a genome-wide fashion. The distribution of these persistent breaks was mostly ascribed to highly transcribed regions of the genome. This approach will now be used to determine how trabectedin induces DNA breaks in genomes of various cancer cell lines to develop TRABI-Seq as a diagnostic tool for cancer therapy.
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Institute for Basic Science
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