Figure 2 (IMAGE)
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COMET Chip assays were used to measure Trabectedin-induced breaks in cells. Each green dot is a single nucleus of a cell and the tail length and the fraction of total DNA in the tail (comet) emerging from each nucleus is proportional to the number of breaks formed. Following UV treatment (left), few breaks are visible as UV lesions are excised from DNA by nucleotide excision repair (NER). Following treatment with trabectedin (center), DNA breaks persist due to an abortive NER reaction. The breaks are dependent on NER as they do not occur in TC-NER deficient cells (right) with inactivated XPF gene.
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Institute for Basic Science
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