Fig. 2: Chemogenetic stimulation of LHb D-neurons regulates dopamine secretion. (IMAGE)
Caption
a Schematic of AAV vectors for Cre-dependent hM3Dq or mCherry expression; representative images of the location of viral injection. b Effect of CNO-induced activation of LHb D-neurons on behaviour observed in the TST as measured by immobility time (n = 14 independent mCherry mice and n = 15 independent hM3Dq mice). c–h Changes in the expression of c-Fos in VTA dopaminergic neurons and RMTg GABAergic neurons caused by chemogenetic stimulation of LHb D-neurons. Representative FISH images in the LHb and medial habenula (MHb) (c) and the percentage of c-Fos-expressing AADC-positive neurons in the LHb by FISH (n = 5 independent mCherry mice and n = 6 independent hM3Dq mice) (d). Expression of c-Fos/TH in the VTA (e) and c-Fos/GAD1 in the RMTg (g) by FISH. Percentage of c-Fos-expressing TH-positive neurons in the VTA (n = 5 independent mCherry mice and n = 6 independent hM3Dq mice) (f) and c-Fos-expressing GAD1-positive neurons in the RMTg by FISH (n = 4 independent mCherry mice and n = 5 independent hM3Dq mice) (h). i, j Effect of chemogenetic stimulation of LHb D-neurons on dopamine release in the NAc. Experimental schematic of microdialysis (i) and quantification of dopamine-release changes pre- and postinjection with CNO (n = 7 independent mCherry mice and n = 5 independent hM3Dq mice), Two-tailed Mann–Whitney U tests were used to compare differences between groups (j). Magnified images of the regions of interest (yellow squares) are shown in (c). Unless otherwise stated, statistical comparisons were performed using a two-tailed unpaired t test. *p < 0.05, **p < 0.01 and ***p < 0.001. Data are presented as the mean values ± s.e.m. Source data are provided as a Source Data file.
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