Fig. 5: LHb D-neurons suppress RMTg GABAergic neurons through trace aminergic signalling. (IMAGE)
Caption
a Schematic of the AAV used and RMTg GABAergic neuronal activity induced by the optogenetic stimulation of LHb D-neurons. b Representative traces (left) and firing frequency in the control mice (middle; n = 10 cells from independent sh-Veh/ChR2 mice) and AADC-knockdown mice (right; n = 13 cells from independent sh-AADC/ChR2 mice) measured at baseline and in the ON or OFF phases of blue-light photoactivation. c, d Expression of GAD1/TAAR1/GAD2 mRNA in GABAergic neurons of the RMTg by FISH (c), and the percentage of TAAR1-expressing GAD1-positive GABAergic neurons in the RMTg by FISH (n = 3 independent mice) (d). e Schematic of AAV injection and RMTg GABAergic neuronal firing rates in response to the application of RO5263397 (RO) or EPPTB. f Representative traces (left) and firing frequency in control (middle; n = 11 cells from independent sh-Veh mice) and AADC-knockdown mice (right; n = 21 cells from independent sh-AADC mice) measured in response to the application of RO5263397 (500 nM) or EPPTB (1 μM). The firing frequency was normalised to the baseline value (b, f). g, h Effect of RO5263397 application in AADC-knockdown mice in vivo on their performance in the TST. Schematic of an AAV in vivo injection (g), and TST immobility time results (n = 7 independent sh-AADC/Saline mice and n = 10 independent sh-AADC/RO mice), a two-tailed unpaired t test was used to compare differences between groups (h). Unless otherwise stated, statistical comparisons were performed using repeated measure one-way ANOVA followed by contrast test. *p < 0.05, **p < 0.01 and ***p < 0.001. Data are presented as the mean values ± s.e.m. Source data are provided as a Source Data file.
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