Lewis Katz School of Medicine at Temple University researchers reveal cellular diversity of esophageal tissue

(Philadelphia, PA) – In a study published today in the prestigious journal Nature Communications, researchers at the Lewis Katz School of Medicine at Temple University defined 11 subsets of cells found in the esophagus of mice, information that could potentially help clinicians diagnose or treat certain types of cancer.

The tissue in the esophagus—known as stratified squamous epithelium—is comprised of a basal layer that ultimately gives rise to several layers of differentiated cells. In this study, researchers sought to find out whether basal cells were all the same or if any differences existed in their gene expressions. 

“This work lays the foundation to address questions about how these cells relate to the development of cancer,” said Kelly Whelan, PhD, senior author on the study and Assistant Professor of Cancer and Cellular Biology and Assistant Professor in the Fels Cancer Institute for Personalized Medicine at the Lewis Katz School of Medicine. 

“The next question we have is if we give a mouse a carcinogen, does one particular subset expand or is another particular subset depleted? If so, we would want to know whether cell types that change in the context of cancer are functionally contributing to the cancer process and whether we can target them to help treat or prevent cancer,” she said.  

To begin to address these questions, Whelan and her fellow researchers used single cell gene-expression profiling, a tool that identifies all the genes in a cell or tissue that make messenger RNA. These profiles can then be used to identify individual cell types within a complex tissue. 

“Essentially, we took more than 40,000 cells from mouse esophageal epithelium and used their individual gene-expression profiles to group them based on their commonality,” she said.

When they started the study, Whelan said the researchers expected to find about three subsets of cells classified as basal, but they actually found six subsets, as well as four that were classified as differentiated. Additionally, one cell type was found to have characteristics of both basal and differentiated cells and was defined as an intermediate cell type.

“We are now working to isolate these individual cell types in order to determine what the cells do functionally in terms of esophageal biology in the normal context first and then in the context of cancer. This study is a fundamental step toward that,” said Whelan.

To read the study, “Single Cell Transcriptomic Analysis Reveals Cellular Diversity of Murine Esophageal Epithelium,” click here. 

About the Lewis Katz School of Medicine
Founded in 1901, the Lewis Katz School of Medicine at Temple University attracts students and faculty committed to advancing individual and population health through culturally competent patient care, research, education, and service. The School confers the MD degree; MS and PhD degrees in Biomedical Science; the MA in Urban Bioethics; the MS in Physician Assistant studies; a certificate in Narrative Medicine; a non-degree post-baccalaureate program; several dual degree programs with other Temple University schools; continuing medical education programs; and in partnership with Temple University Hospital, 40 residency and fellowship programs for physicians. The School also manages a robust portfolio of publicly and privately funded transdisciplinary studies aimed at advancing the prevention, diagnosis, and treatment of disease -- with specialized research centers focused on heart disease, cancer, substance use disorder, metabolic disease, and other regional and national health priorities. To learn more about the Lewis Katz School of Medicine, please visit: medicine.temple.edu.