image: Multiphoton images of human skin at a depth of 15-20 μm below the skin surface in volunteers after application of uncoated and silicone-coated zinc oxide nanoparticles suspended in a commercial sunscreen base compared with no product (untreated control). Red and yellow pseudocolor indicates the presence of zinc oxide nanoparticles in skin furrows. The blue-green color shows autofluorescence from living epidermal cells, with no evidence of zinc oxide nanoparticle penetration. view more
Credit: <i>Journal of Investigative Dermatology</i>
Berlin, December 4, 2018 - Zinc oxide (ZnO) has long been recognized as an effective sunscreen agent. However, there have been calls for sunscreens containing ZnO nanoparticles to be banned because of potential toxicity and the need for caution in the absence of safety data in humans. An important new study provides the first direct evidence that intact ZnO nanoparticles neither penetrate the human skin barrier nor cause cellular toxicity after repeated application to human volunteers under in-use conditions. This confirms that the known benefits of using ZnO nanoparticles in sunscreens clearly outweigh the perceived risks, reports the Journal of Investigative Dermatology.
The safety of nanoparticles used in sunscreens has been a highly controversial international issue in recent years, as previous animal exposure studies found much higher skin absorption of zinc from application of ZnO sunscreens to the skin than in human studies. Some public advocacy groups have voiced concern that penetration of the upper layer of the skin by sunscreens containing ZnO nanoparticles could gain access to the living cells in the viable epidermis with toxic consequences, including DNA damage. A potential danger, therefore, is that this concern may also result in an undesirable downturn in sunscreen use. A 2017 National Sun Protection Survey by the Cancer Council Australia found only 55 percent of Australians believed it was safe to use sunscreen every day, down from 61 per cent in 2014.
Investigators in Australia studied the safety of repeated application of agglomerated ZnO nanoparticles applied to five human volunteers (aged 20 to 30 years) over five days. This mimics normal product use by consumers. They applied ZnO nanoparticles suspended in a commercial sunscreen base to the skin of volunteers hourly for six hours and daily for five days. Using multiphoton tomography with fluorescence lifetime imaging microscopy, they showed that the nanoparticles remained within the superficial layers of the stratum corneum and in the skin furrows. The fate of ZnO nanoparticles was also characterized in excised human skin in vitro. They did not penetrate the viable epidermis and no cellular toxicity was seen, even after repeated hourly or daily applications typically used for sunscreens.
"The terrible consequences of skin cancer and photoaging are much greater than any toxicity risk posed by approved sunscreens," stated lead investigator Michael S. Roberts, PhD, of the Therapeutics Research Centre, The University of Queensland Diamantina Institute, Translational Research Institute, Brisbane, and School of Pharmacy and Medical Sciences, University of South Australia, Sansom Institute, Adelaide, QLD, Australia.
"This study has shown that sunscreens containing nano ZnO can be repeatedly applied to the skin with minimal risk of any toxicity. We hope that these findings will help improve consumer confidence in these products, and in turn lead to better sun protection and reduction in ultraviolet-induced skin aging and cancer cases," he concluded.
"This study reinforces the important public health message that the known benefits of using ZnO nano sunscreens clearly outweigh the perceived risks of using nano sunscreens that are not supported by the scientific evidence," commented Paul F.A. Wright, PhD, School of Health and Biomedical Sciences, RMIT University, Bundoora, VIC, Australia, in an accompanying editorial. "Of great significance is the investigators' finding that the slight increase in zinc ion concentrations in viable epidermis was not associated with cellular toxicity under conditions of realistic ZnO nano sunscreen use.
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Journal
Journal of Investigative Dermatology