video: Dissociation of StrepTactin and monomerized MHC from individual living T cells with overlayed fluorescence channels. One representative movie demonstrating the changes in fluorescence intensities after the addition of D-biotin to the cells. The APC fluorescence (blue) is rapidly lost during the dissociation of Strep-Tactin while Atto565 fluorescence intensity (red) increases, followed by a decrease until complete loss of Atto565 fluorescence. This video relates to a paper that appeared in the July 3, 2013, issue of Science Translational Medicine, published by AAAS. The paper, by M. Nauerth at Technische Universität München in Munich, Germany, and colleagues was titled, “TCR-Ligand koff-Rate Correlates with the Protective Capacity of Antigen-Specific CD8+ T Cells for Adoptive Transfer.” view more
Credit: Video courtesy of <i>Science Translational Medicine</i>/AAAS