ELSI scientist constructs artificial photosynthetic cells

A team led by associate professor Yutetsu Kuruma of the Earth-Life Science Institute (ELSI) at Tokyo Institute of Technology has constructed simple artificial cells that can produce chemical energy that helps synthesize parts of the cells themselves. This work marks an important milestone in constructing fully photosynthetic artificial cells, and may shed light on how primordial cells used sunlight as an energy source early in life's history.

Scientists build artificial cells as models of primitive cells, as well as to understand how modern cells function. Many sub-cellular systems have now been built by simply mixing cell components together. However, real living cells construct and organize their own components. It has also been a long time goal of research to build artificial cells that can also synthesize their own constituents using the energy available in the environment.

The Tokyo Tech team combined a cell-free protein synthesis system, which consisted of various biological macromolecules harvested from living cells, and small protein-lipids aggregates called proteoliposomes, which contained the proteins ATP synthase and bacteriorhodopsin, also purified from living cells, inside giant synthetic vesicles. ATP synthase is a biological protein complex that uses the potential energy difference between the liquid inside a cell and the liquid in the cell's environment to make the molecule adenosine triphosphate (ATP), which is the energy currency of the cell. Bacteriorhodopsin is a light-harvesting protein from primitive microbes that uses light energy to transport hydrogen ions outside of the cell, thus generating a potential energy difference to help ATP synthase operate. Thus, these artificial cells would be able to use light to make a hydrogen ion gradient that would help make the fuel cells use to run their sub-cellular systems, including making more protein.

Just as the scientists hoped, the photosynthesized ATP was consumed as a substrate for transcription, the process by which biology makes messenger RNA (mRNA) from DNA, and as an energy for translation, the process by which biology makes protein from mRNA. By also including the genes for parts of the ATP synthase and the light-harvesting bacteriorhodopsin, these processes also eventually drive the synthesis of more bacteriorhodopsin and the constituent proteins of ATP synthase, a few copies of which were included to "jump-start" the proteoliposome. The newly formed bacteriorhodopsin and ATP synthase parts then spontaneously integrated into the artificial photosynthetic organelles and further enhanced ATP photosynthesis activity.

As professor Kuruma states "I have been trying for a long time to construct a living artificial cell, especially focusing on membranes. In this work, our artificial cells were wrapped in lipid membranes, and small membrane structures were encapsulated inside them. In this way, the cell membrane is the most important aspect of forming a cell, and I wanted to show the importance of this point in the study of artificial cell and feedback in origins of life studies."

Kuruma thinks the most impact point of this work is that artificial cells can produce energy to synthesize the parts of the cell itself. This means that the artificial cells could be made to be energetically independent and then it would be possible to construct self-sustaining cells, just like actual biological ones cells. "The most challenging thing in this work was the photosynthesis of the bacteriorhodopsin and the ATP synthase parts, which are membrane proteins. We tried to photosynthesize a full ATP synthase, which has 8 kinds of component proteins, but we could not because of the low productivity of the cell-free protein synthesis system. But, if it was upgraded, we may photosynthesize the whole 8 kinds component proteins."

Nevertheless, this work demonstrates that a simple biologically inspired system including two kinds of membrane protein is able to supply energy to drive gene expression inside a microcompartment. Thus, primordial cells using sunlight as a primal energy source could have existed early in life's evolution before modern autotrophic cells arose. The team believes attempts to construct living artificial cells will help understand the transition from non-living to living matter that took place on early Earth and, help develop biology-based devices that can sense light and drive biochemical reactions. These artificial photosynthetic cell systems also help pave the way to constructing energetically independent artificial cells.

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Reference

Samuel Berhanu¹, Takuya Ueda¹, Yutetsu Kuruma^2,3, Artificial photosynthetic cell producing energy for protein synthesis, Nature Communications, DOI: 10.1038/s41467-019-09147-4

1. University of Tokyo, Japan
2. Earth-Life Science Institute, Tokyo, Japan
3. JST PRESTO, Japan

Tokyo Institute of Technology (Tokyo Tech)

Tokyo Tech stands at the forefront of research and higher education as the leading university for science and technology in Japan. Tokyo Tech researchers excel in fields ranging from materials science to biology, computer science, and physics. Founded in 1881, Tokyo Tech hosts over 10,000 undergraduate and graduate students per year, who develop into scientific leaders and some of the most sought-after engineers in industry. Embodying the Japanese philosophy of “monotsukuri,” meaning “technical ingenuity and innovation,” the Tokyo Tech community strives to contribute to society through high-impact research. http://www.titech.ac.jp/english/

The Earth-Life Science Institute (ELSI)

Launched in 2012, ELSI is one of Japan’s ambitious World Premiere International research centers, whose aim is to achieve progress in broadly inter-disciplinary scientific areas by inspiring the world’s greatest minds to come to Japan and collaborate on the most challenging scientific problems. ELSI’s primary aim is to address the origin and co-evolution of the Earth and life.

WPI

The World Premier International Research Center Initiative (WPI) was launched in 2007 by the Ministry of Education, Culture, Sports, Science and Technology (MEXT) to help build globally visible research centers in Japan. These institutes promote high research standards and outstanding research environments that attract frontline researchers from around the world. These centers are highly autonomous, allowing them to revolutionize conventional modes of research operation and administration in Japan.

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