image:
Effects of L. draba extract on p53-positive cells in (A) kidney and liver tissues (DAB staining 400×, scale bar = 25 μm). (B) Quantitative results of immunohistochemical examination. Data are shown as mean ± SEM (n=6). **P<0.001 compared with the SC group; #P<0.05, ##P<0.001 compared with the CP group. p53-positive cells are stained brown.
view moreCredit: Yu-Lai You, Sheng Zheng, Cheng-Jian Zhao, Ye-Fei Chang, Pei Liu, Xue-Li Zeng, Lian Liu
Cyclophosphamide (CP) is used directly or through its metabolites (phosphoramide mustard) to treat a variety of tumors. However, it has adverse effects. Lepidium draba L. (L. draba) has been used to treat many diseases. The current study aimed to examine the protective effects of L. draba on the liver and kidney in rats of CP-induced toxicity.
A total of 36 rats were divided into six groups as follows: the sham control group, the CP group (CP 100 mg/kg i.p. on days 1, 7, 14, 21, 28, and 35), the CP groups treated with L. draba extract (100, 200 and 400 mg/kg of L. draba extract for 28 d), and the L. draba extract alone group (400 mg/kg of L. draba extract for 28 d). Serum parameters of renal and hepatic function, as well as pro-inflammatory and anti-inflammatory cytokines associated with liver and kidney damage were measured. Moreover, Bax, Bcl-2, and caspase-3 gene expression and histopathological changes were assessed.
L. draba extract alleviated CP-induced hepatotoxicity and nephrotoxicity by decreasing nitric oxide, TBARS, IL-6, TNF-α, and IL-1β levels, as well as increasing superoxide dismutase, catalase and glutathione peroxidase activities, and FRAP, MIF, and TGF-β levels. In addition, the extract downregulated the expression of pro-apoptotic genes (Bax and caspase-3) and mitigated the destruction of glomeruli and renal tubules as well as the degeneration of hepatocytes.
L. draba extract can protect hepatic and renal structure and function against CP-induced toxicities, and may be used as a therapeutic agent for CP-induced hepatotoxicity and nephrotoxicity.
The work entitled “Hepatorenoprotective effects of Lepidium draba L. extracts against cyclophosphamide-induced oxidative injuries in rats via reducing apoptosis and inflammation” was published on Asian Pacific Journal of Tropical Biomedicine (published on Feb. 27, 2025).
DOI：10.4103/apjtb.apjtb_518_24
Journal
Asian Pacific Journal of Tropical Biomedicine
Method of Research
Experimental study
Subject of Research
Animals
Article Title
Hepatorenoprotective effects of Lepidium draba L. extracts against cyclophosphamide-induced oxidative injuries in rats via reducing apoptosis and inflammation
Article Publication Date
27-Feb-2025