image: From left, Utah State University researchers Emily Calhoun, doctoral student, and Norah Saarman, assistant professor in the Department of Biology and USU Ecology Center, retrieve mosquito specimens from a lab freezer. Saarman is the recipient of a grant from the American Mosquito Association Research Fund to aid her efforts in developing AI-based species identification tools to mitigate the spread of mosquito-borne diseases.
Credit: M. Muffoletto, USU
LOGAN, UTAH, USA — Morphology is the study of the form and structure of organisms, including their physical characteristics such as shape, size and arrangement of parts. Morphology is key to taxonomy, the science of classifying organisms, as scientists use morphology to identify and study species, as well as to explore evolutionary processes.
Identifying species is challenging — even with large animals and plants, says Utah State University ecologist Norah Saarman.
“Trying to visually identify different species in small organisms such as mosquitoes is extremely difficult, as the species are very similar and their body parts are so tiny,” Saarman said. “Even with magnification and training, efforts to accurately identify a specific species are often inconclusive.”
Yet accurate identification is crucial in attempts to identify disease vectors and how to control their spread.
To this end, Saarman, assistant professor in the Department of Biology and USU Ecology Center, was awarded a $54,000 grant from the American Mosquito Association Research Fund toward her efforts to develop tools for efficient, low-cost and accurate identification of Culex mosquito vectors of West Nile Virus using computer vision-based AI technology.
Utah isn’t among the worst U.S. states for mosquitoes, yet varied species make themselves at home in the Beehive state. Among these pesky fliers is the Northern House Mosquito, Culex pipiens, which can spread West Nile Virus and St. Louis encephalitis in humans, as well as avian malaria in birds and heartworm disease in dogs.
Newer to northern Utah is the Southern House Mosquito, Culex quinquefasciatus, also known as “Quinx,” which is very similar to Culex pipiens, is a vector for many of the same diseases and is better at spreading West Nile Virus. Quinx was first identified in southern Utah in the 1950s but has made its way to the Salt Lake City area in recent years.
“Southern House Mosquitoes are a more effective vector of West Nile Virus because they are more likely to bite mammals and transmit the virus from birds to mammals,” Saarman says. “They are also a public health concern because they are known to be more capable of evolving resistance to commonly used insecticides.”
Additionally, Quinx breed with the Northern House Mosquito, producing hybrid populations.
“We need better identification tools to monitor populations of these insects, along with hybrid populations resulting from interbreeding,” Saarman says.
She has long collaborated with the Salt Lake City Mosquito Abatement District and the Utah Department of Health and Human Services to study and monitor the state’s mosquito populations. With mosquitoes collected and supplied by these entities, Saarman and her team members are refining methods of identifying species with morphological techniques and DNA testing. They’ll combine these methods with machine learning tools supplied by industry collaborator Vectech, Inc. to develop an AI method for identifying species with increased efficiency, accuracy and cost-effectiveness.
“Tracking mosquitoes and disease outbreaks is challenging, because mosquito populations rise and fall throughout the season, and they move and adapt quickly to changing conditions,” she says. “As Utah becomes increasingly urbanized, mosquitoes flock to welcoming habitats, such as storm drain catchment basins, unintentionally created by human developments.”
Mosquitoes need at least two things for survival: blood from vertebrate hosts and water. Try as you might to avoid these insects, your human blood and your animal companions, including dogs, cats and chickens, along with other wild animals attracted to human habitats such as squirrels, rats, mice and birds — as well as your human water-consuming habits — are irresistible attractions.
Saarman says female mosquitoes require a blood meal from a human, animal or bird to provide the energy needed to successfully lay their eggs directly on or near water. Even in arid regions, catch basins and storm drains, installed where humans reside and designed to collect runoff from creeks, streams and irrigation, create ideal breeding habitat for mosquito larvae.
“We think of Utah as a dry state and assume our hot summers will dry up pooled water, yet some infrastructure creates reservoirs of stagnant water — so small, you wouldn’t think they are problem — that are just right for mosquitoes,” she says.
Urban managers use larvicide, including Bacillus spaericus, to quickly wipe out mosquito larvae before they emerge to find blood hosts, yet judicious use is crucial.
“These larvicides are generally safe for humans, livestock, pets and non-target insects in managed doses,” Saarman says. “But mosquito larvae evolve a resistance to them. Being able to monitor the level of resistance, in which species it’s occurring and where it’s taken place is a management challenge. The ability to accurately and more quickly identify species will aid this challenge.”
Utah reported 14 West Nile Virus cases in humans, one resulting in death, in 2024, which might seem a small number.
“The Utah Department of Health and Human Services reports an average of 25 human cases per year in the state, and we shouldn’t overlook a surge of 158 cases logged during a high transmission event in summer 2008,” Saarman says. “Even in years with few cases, West Nile Virus is a serious neuroinvasive disease that can progress to dangerous and lingering complications, including meningitis, encephalitis and Acute Flaccid Paralysis.”
She and her team’s focus is on preventing vector-borne disease in the safest, most cost-effective and environmentally friendly ways possible.
“Doing that means we need to know which species we’re dealing with and the pathogens they’re infected with as soon as possible,” Saarman says. “The power of AI will help us achieve this.”
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