Identifying the neural mechanisms regulating sequential behaviors in male sexual activity

Tsukuba, Japan—Male mice follow a stereotypical sequence of sexual behaviors, transitioning from initial interaction with an estrous female to ejaculation. This sequence includes sniffing, mounting, and intromission, all of which are crucial for reproductive success. However, the specific brain mechanisms underlying these behavioral transitions remained unclear until now.

In this study, researchers demonstrated that dopamine, a key neurotransmitter in the brain's reward system, plays a pivotal role in governing behavioral shifts. Using fiber photometry, they comprehensively measured dopamine input patterns to the nucleus accumbens, a region known for its substantial dopamine input. Their findings showed that dopamine levels in a specific subregion—the ventral shell of the nucleus accumbens (vsNAc)—were closely linked to behavioral transitions. Furthermore, rhythmic dopamine input to the vsNAc during intromission, which aligns with male movement patterns, was regulated by acetylcholine innervation of the vsNAc.

The study also revealed that the final intromission before ejaculation exhibited a distinct dopamine activity pattern compared to prior intromissions. Artificially stimulating acetylcholine neurons during intromission replicated this dopamine response, directly triggering ejaculation.

These findings provide the first evidence of a specific nucleus accumbens subregion playing a critical role in controlling sexual behavior and ejaculation. Ejaculatory dysfunction is a known side effect of psychiatric disorders such as depression, as well as certain psychotropic medications. These discoveries open new avenues for developing treatments for ejaculatory disorders and related conditions, potentially improving therapies for patients with psychiatric disorders or medication-induced dysfunction.

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This study was supported by JSPS KAKENHI (JP21J22555 and JP24K23231 to A.M.), JSPS KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas, "Willdynamics" (JP19H05006 to K.S.); AMED under Grant JP21zf0127005 (to Q.L.); the WPI program from Japan's MEXT, and the New Cornerstone Science Foundation.

_{Original Paper}

Title of original paper:
Sequential Transitions of Male Sexual Behaviors Driven by Dual Acetylcholine-Dopamine Dynamics

Journal:
Neuron

DOI:
10.1016/j.neuron.2025.01.032

_{Correspondence}

Professor SAKURAI, Takeshi
Institute of Medicine / International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba

SEITA, Jun MD, Ph. D
Team Leader, Advanced Data Science Project, RIKEN Information R&D and Strategy Headquarters, RIKEN

_{Related Link}

Institute of Medicine

International Institute for Integrative Sleep Medicine (WPI-IIIS)

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About International Institute for Integrative Sleep Medicine (IIIS), University of Tsukuba
World-class institute for sleep medicine, aiming to solve the mechanism of sleep/wakefulness by conducting basic to translational research
The mission of IIIS is to be a multidisciplinary, international hub for the research to elucidate the function of sleep and the fundamental mechanisms of sleep/wake regulation, to elucidate molecular pathogenesis of sleep disorders and related diseases, to develop preventive measures, diagnostic methods, and treatments for sleep.