New immune-probing technique could boost treatment discovery

Osaka, Japan – Developing effective treatments and understanding how the mechanism of the immune system have always been challenging for scientists. T cells, which are the frontline warriors in the battle against the infections, have been a major focus of research. Regulatory T cells (Tregs) are specialized immune cells that have a challenging task in the body. They must balance the immune system by making it aggressive enough to fight infection and cancer but calm enough not to attack the body’s normal cells. Tregs meet this challenge by controlling the actions of other immune cells. However, we don’t know exactly how these cells interact during disease and treatment.

In a study recently published in Nature Communications, a research team at Osaka University has developed a new technique that reveals how Tregs exert their control. The new technique, called single-cell suppressive profiling of regulatory T cells (scSPOT), can pinpoint the effects of Tregs on all other immune cells simultaneously. Previously, researchers could only observe interactions between one or two cell types at a time.

“This new method provides a better understanding of how Tregs function in a complex immune environment in a way that closely resembles what happens in the human body,” explains first author of the study Jonas Nørskov Søndergaard. “Using scSPOT, we found that Tregs most strongly affect CD8-EM T cells by suppressing their ability to grow and divide.”

CD8-EM T cells are a subset of memory T cells that have a key role in fighting cancer and infections, highlighting how Tregs are closely tied with health and disease. The team also found that Tregs are targets for the cancer drugs ipilimumab and tazemetostat, although the two drugs affect Tregs in different ways. Tregs are indicators of serious viral infection too, since scSPOT identified special types of Tregs like the ones seen in patients with severe COVID-19 infection.

“Our new scSPOT method is a valuable tool that may accelerate the development of treatments for cancer and other diseases,” says senior author James Wing. “In particular, the discovery of indicators of severe viral infections may allow healthcare professionals to identify high-risk patients earlier, allowing for faster treatment during outbreaks and pandemics."

The team’s scSPOT method has already boosted our understanding of the immune system. The insights it provides into the ways existing drugs work could help investigators develop more effective treatment strategies, leading to better outcomes for patients.

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The article, “Single cell suppression profiling of human regulatory T cells,” was published in Nature Communications at DOI: https://doi.org/10.1038/s41467-024-55746-1

About Osaka University
Osaka University was founded in 1931 as one of the seven imperial universities of Japan and is now one of Japan's leading comprehensive universities with a broad disciplinary spectrum. This strength is coupled with a singular drive for innovation that extends throughout the scientific process, from fundamental research to the creation of applied technology with positive economic impacts. Its commitment to innovation has been recognized in Japan and around the world. Now, Osaka University is leveraging its role as a Designated National University Corporation selected by the Ministry of Education, Culture, Sports, Science and Technology to contribute to innovation for human welfare, sustainable development of society, and social transformation.
Website: https://resou.osaka-u.ac.jp/en