image: (a) Total ion current chromatograms (TIC) of SQ extract by ultra-performance liquid chromatography-triple quadrupole time-of-flight mass spectrometry (UPLC-Triple-TOF/MS), negative ion mode; (b) TIC of SQ extract by UPLC-Triple-TOF/MS, positive ion mode; (c) Pie chart of the number of different types of compounds in SQ extract; (d) Pie chart of the number of compounds from different herbs in SQ extract. AM, Radix Astragali; CP, Radix Codonopsis pilosula.
Credit: Xiaohui Fan, Xiaohui Fan, Menglei Wang, Bingjie Zhu
Background and objectives
Shenqi Fuzheng (SQ) is a widely used Chinese medicine formula known for its immune-enhancing and Qi-supplementing properties. However, the blood-absorbed components of SQ and their pharmacokinetics remain underexplored. This study aimed to comprehensively analyze the chemical constituents of SQ and investigate their absorption and pharmacokinetic behavior in rat plasma.
Methods
Ultra-performance liquid chromatography-triple quadrupole time-of-flight mass spectrometry (hereinafter referred to as UPLC-Triple-TOF/MS) is employed to identify the chemical components in SQ extract and quantify the components absorbed into the blood after oral administration in rats. This method provides fragmentation patterns of compounds and key pharmacokinetic profiles of blood-absorbed compounds.
Results
A total of 105 compounds are identified from the SQ extract, and 40 are detected in the blood following oral administration. Organic acids and amino acids are found at higher concentrations in the bloodstream. Compounds such as Astragalosides promptly enter the bloodstream within 5 m after administration, with levels declining after 15 m. Flavonoids are absorbed within 15–30 m, and the peak of alkaloids occurs approximately 1 h after administration.
Conclusions
This study provides new insights into the chemical composition and pharmacokinetics of SQ, highlighting the dynamic changes in the content of absorbed compounds in the blood. It further promotes the comprehensive characterization of traditional Chinese medicine formulations through UPLC-Triple-TOF/MS. Future research should focus on elucidating the pharmacological activities of the identified compounds and investigating their potential synergistic effects within the formulation.
Full text:
https://www.xiahepublishing.com/2835-6357/FIM-2024-00037
The study was recently published in the Future Integrative Medicine.
Future Integrative Medicine (FIM) publishes both basic and clinical research, including but not limited to randomized controlled trials, intervention studies, cohort studies, observational studies, qualitative and mixed method studies, animal studies, and systematic reviews.
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Journal
Future Integrative Medicine
Article Title
Identification of Chemical Constituents and Blood-absorbed Components of Shenqi Fuzheng Extract Based on UPLC-Triple-TOF/MS Technology
Article Publication Date
4-Dec-2024