Historic outbreaks of coffee wilt disease linked to gene transfer from another fungus

The fungus that causes coffee wilt disease repeatedly took up segments of DNA from a related fungal pathogen, which contributed to successive outbreaks of the disease. Lily Peck of Imperial College London, UK, reports these new findings in a study published December 5^th in the open-access journal PLOS Biology.

During the past century, outbreaks of coffee wilt disease, which are caused by the fungal pathogen Fusarium xylarioides, have severely impacted coffee production across sub-Saharan Africa. Despite being discovered in 1927, little is known about the pathogen’s genetics and evolution, and this understanding is crucial for managing future outbreaks.

In the new study, researchers compared the genomes of 13 historic strains spanning six decades and multiple disease outbreaks to investigate how the pathogen has evolved and adapted to its host plants. They found that F. xylarioides is made up of at least four distinct lineages: one specialized to live on arabica coffee plants, one adapted to robusta coffee plants and two historic lineages that infected multiple coffee species. The researchers also found evidence that these strains had repeatedly received segments of DNA from another fungal pathogen, F. oxysporum, which enhanced F. xylarioides’s ability to infect coffee plants. These additions of DNA, called horizontal gene transfer, likely contributed to the repeated emergence of coffee wilt disease on the African continent.

Previous studies have shown that similar horizontal gene transfer events contributed to new outbreaks of other pathogens, including potato blight, certain wheat fungal pathogens and Aspergillus fumigatus, a fungus that most commonly infects immunocompromised individuals. The researchers concluded that the use of genomics techniques on historical strains of fungi, sampled during the last century and held in culture collections, would provide a powerful way to further investigate the role of horizontal gene transfer in fungal outbreaks.

The authors add, “An historical approach shows the importance of horizontal transfers and large mobile elements in the emergence of new types of disease. If we can understand how new types of diseases evolve, we can give growers the knowledge they need to reduce the risk of new diseases emerging in the first place.”

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In your coverage, please use this URL to provide access to the freely available paper in PLOS Biology: http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3002480

Citation: Peck LD, Llewellyn T, Bennetot B, O’Donnell S, Nowell RW, Ryan MJ, et al. (2024) Horizontal transfers between fungal Fusarium species contributed to successive outbreaks of coffee wilt disease. PLoS Biol 22(12): e3002480. https://doi.org/10.1371/journal.pbio.3002480

Author Countries: United Kingdom, France

Funding: This research was supported by the Natural Environment Research Council [Grant number NE/L002515/1 (LDP) and NE/S010866/1 (TGB).] The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.