Pathogens which cling to microplastics may survive wastewater treatment

Wastewater treatment fails to kill several human pathogens when they hide out on microplastics in the water, reports a new study led by Ingun Lund Witsø of the Norwegian University of Life Sciences, published November 6, 2024 in the open-access journal PLOS ONE.

Wastewater treatment plants are designed to remove contaminants from wastewater, but microplastics persist and can become colonized by a sticky microbial biofilm. Previous research has suggested that these microbial communities, called plastispheres, include potential pathogens, and thus might pose a risk to human health and the environment when treated wastewater and sludge are released.

In the new study, researchers identified food-borne pathogens in plastispheres living on three types of plastic in wastewater. They cultured the microorganisms and used genetic techniques to understand the diversity and members of the plastisphere communities. The team found evidence of pathogenic bacteria and viruses, including Listeria monocytogenes, Escherichia coli, norovirus and adenovirus. They also successfully grew Klebsiella pneumoniae and Acinetobacter spp. from raw and treated wastewater, indicating that the plastisphere biofilms likely protect the pathogens from wastewater treatment.

These findings highlight the potential of plastispheres to harbor and spread pathogens, which poses a challenge to safely reusing wastewater. Without efficient wastewater treatment and plastic waste management, wastewater could act as a vehicle for transferring plastic-associated pathogens into the food chain. The researchers emphasize that continued research and innovation are essential to remove microplastics – and their pathogens – from wastewater.

The authors add: “Plastics in wastewater treatment plants are colonized by microbial biofilms, or “plastispheres,” which can harbor pathogens, including Listeria, E. coli, Klebsiella pneumoniae, and Acinetobacter spp., that persist through treatment processes. This study highlights the potential for plastispheres to contribute to the spread of pathogens from treated wastewater, posing challenges for environmental health and water reuse efforts.”

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In your coverage please use this URL to provide access to the freely available article in PLOS ONE: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0312157

Citation: Witsø IL, Basson A, Aspholm M, Wasteson Y, Myrmel M (2024) Wastewater-associated plastispheres: A hidden habitat for microbial pathogens? PLoS ONE 19(11): e0312157. https://doi.org/10.1371/journal.pone.0312157

Author Countries: Norway

Funding: This work was supported by The Norwegian Research Council grant (PLASTPATH project number 302996). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.