image: (A) The chemical structures of Ru1–Ru9. (B) Workflow for molecular target identification and mechanisms investigation of Ru5 using the photo-affinity probe Ru5a by photoaffinity-based protein profiling.
Credit: ©Science China Press
This study is led by Prof. Cai-Ping Tan, Prof. Zong-Wan Mao (MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University) and Prof. Zheng-Qiu Li (School of Pharmacy, MOE Key Laboratory of Tumor Molecular Biology, Jinan University). The team identified Ru5 as a potent antitumor agent by screening a panel of ruthenium(II) polypyridine complexes containing β-carboline derivatives as ligands. By employing a photoaffinity tag and utilizing the photoaffinity-based protein profiling technology, the researchers successfully elucidated mitochondrial ATPase as the primary molecular target of Ru5.
As an inhibitor of ATPase, Ru5 induces mitochondrial dysfunction, autophagy, and ferroptosis, which collectively contribute to its antitumor efficacy. Furthermore, comprehensive multi-omics analyses unveil the mechanism by which Ru5 activates the ferroptosis pathway via modulation of chloride channel protein expression, alternation in mitochondrial permeability, and elevation of reactive oxygen species levels. Additionally, Ru5 effectively suppresses the expression of epithelial-mesenchymal transition-related genes associated with cancer migration and invasion. In vivo studies using a human lung cancer (A549) xenografted nude mouse model demonstrate superior tumor inhibition by Ru5 compared to the clinical drug cisplatin without causing significant weight loss or organ damage during treatment.
This study not only offers novel insights into the mechanisms of action of ruthenium(II) polypyridine complexes in anticancer therapy but also presents innovative strategies for developing new metallo anticancer agents by integrating photoaffinity labeling techniques and multi-omics approaches. Through comprehensive molecular target investigations, the work provides a crucial theoretical basis for enhancing treatment efficacy, minimizing side effects, overcoming drug resistance and evaluating the safety and effectiveness of metal-based anticancer agents.
###
See the article:
Identification of mitochondrial ATP synthase as the cellular target of Ru-polypyridyl-β-carboline complexes by affinity-based protein profiling
https://doi.org/10.1093/nsr/nwae234
Journal
National Science Review