Scientists Learn How to Drug Wily Class of Disease-Causing Enzymes
Drugs for the K-Ras oncogene inspire an approach for targeting the GTPases, a family of enzymes whose dysfunction can lead to Parkinson’s and many other diseases.
UCSF scientists have discovered how to target a class of molecular switches called GTPases that are involved in a myriad of diseases from Parkinson’s to cancer and have long been thought to be “undruggable.”
Because of their slippery exteriors, the GTPases have remained largely out of reach of modern drug discovery, with the exception of the notorious cancer-causing GTPase called K-Ras.
On a hunch, the team tested a dozen drugs that target K-Ras against a handful of GTPases they had mutated to make them more receptive to the drugs. The approach revealed new drug binding sites that could not have been predicted by computational drug discovery tools.
The findings, published on Sept 9 in Cell, create a long-awaited opportunity to develop therapies for the many, diverse diseases that arise from GTPase dysfunction.
“We’ve known about the GTPases for decades but have lacked any way to reliably drug them,” said Kevan Shokat, PhD, UCSF professor in the Department of Cellular and Molecular Pharmacology and senior author of the paper. “This really puts all those GTPases on the map for drug discovery, so it’s possible to target them when they’re associated with disease.”
Our cells depend on networks of GTPases, which oversee everything from the movement of molecules to cell growth and division. When something goes wrong among these switches, disease can follow.
In 2013, Shokat and colleagues discovered a “pocket” where drugs could bind to K-Ras, a famous GTPase that’s responsible for up to 30% of all cancer cases.
Since then, nearly a dozen drugs have been developed targeting mutations to K-Ras, but the other GTPases remained untouchable.
In the present work, Shokat’s team, led by UCSF postdoctoral scholar and first author Johannes Morstein, PhD, engineered one of the cancer-causing K-Ras mutations, G12C, into a representative group of GTPases.
They guessed that G12C, which places a chemical “hook” onto a protein, could help them fish out which among ten K-Ras G12C drugs might bind to other GTPases, which have only subtle similarity to K-Ras itself.
The laboratory experiments turned up gold: with the help of G12C, some of the K-Ras drugs bound to the otherwise featureless GTPases. When G12C was removed, those drugs still bound to the GTPase.
The approach, dubbed chemical genetics, exploited the flexibility of the GTPases, enabling the drugs to nudge open a pocket in the protein where it could lodge itself. This pocket had evaded previous efforts to predict, computationally, where drugs might bind.
“Since these GTPases switch between ‘on’ and ‘off’ states, the pocket is not usually visible, certainly not to the standard software used for drug discovery,” Shokat said. “Instead, the drug binds to an intermediate state, freezing the GTPases and inactivating them.”
The researchers are sharing their methods openly in the hopes that others will use them to drug their GTPase of interest, whether it’s a Rab GTPase, which is implicated in Alzheimer’s, or a Rac GTPase, which plays a role in breast cancer. Among the hundreds of GTPases, there’s rich potential to make progress for patients.
“In the case of these enzymes, it was critical for us to first test our ideas experimentally in the laboratory, to actually see what worked,” Morstein said. “We’re hopeful it can really accelerate drug discovery.”
Other authors: Critical contributions were made by researchers at Lawrence Livermore National Lab and the National Cancer Institute’s RAS initiative. Other UCSF authors are Victoria Bowcut, Micah Fernando, Lawrence Zhu, Keelan Z Guiley, Matthew Peacock, Zhi Lin, Katrine A. Taran, and Benjamin J Huang. For all authors, see the paper.
Funding and disclosures: The work was supported by in part by federal grants (K99CA277358, 5R01CA244550, NSERC-2020-04241, 75N91019D00024). Several authors are inventors on patents owned by UCSF covering GTPase-targeting small molecules. For all funding and disclosures, see the paper.
About UCSF: The University of California, San Francisco (UCSF) is exclusively focused on the health sciences and is dedicated to promoting health worldwide through advanced biomedical research, graduate-level education in the life sciences and health professions, and excellence in patient care. UCSF Health, which serves as UCSF's primary academic medical center, includes top-ranked specialty hospitals and other clinical programs, and has affiliations throughout the Bay Area. UCSF School of Medicine also has a regional campus in Fresno. Learn more at ucsf.edu, or see our Fact Sheet.
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