Chloroplast genome study separates C. meiocarpa and C. oleifera to enhance tea-oil camellia breeding and genetic research

A research team sequenced and analyzed the complete chloroplast genomes of C. oleifera and C. meiocarpa, uncovering significant differences that clarified their phylogenetic relationship. This study developed 17 chloroplast DNA primers for distinguishing between them, contributing valuable tools for assessing genetic diversity in tea-oil Camellia. These findings hold significant potential for future research and practical applications in germplasm resource assessment, molecular phylogenetic analysis, and the innovation and utilization of tea-oil Camellia in agricultural and industrial practices.

Tea-oil Camellia, known for its high oil content and health benefits, is a key woody oil crop with significant cultivation value, especially in China. Despite its importance, the phylogenetic relationships among tea-oil Camellia species, particularly between C. meiocarpa and C. oleifera, remain controversial due to complex nuclear genomes and interspecific hybridization. Moreover, no reports exist on the chloroplast genome of C. meiocarpa, and no comparative analysis has been done between C. oleifera and C. meiocarpa. These unresolved issues hinder effective breeding and production.

A study (DOI: 10.48130/tp-0024-0022) published in Tropical Plants on 24 July 2024, aims to clarify these relationships by analyzing the chloroplast genomes of C. oleifera and C. meiocarpa and developing molecular markers for variety identification and resource utilization.

The research employed comparative genomics to analyze the chloroplast genomes of C. meiocarpa (CKX/XG) and C. oleifera (HZP), focusing on genome structures, repetitive sequences, IR region variations, nucleotide diversity, and phylogenetic relationships. The chloroplast genomes exhibited a typical circular tetramerous structure with slight size differences among the species. Repetitive sequences and simple sequence repeats (SSRs) were distributed unevenly, with distinct patterns observed between the species. The expansion and contraction of the IR regions revealed genomic adaptations, particularly between HZP and CKX/XG. Nucleotide diversity analysis highlighted the ycf1 gene as having the most mutations, and intergenic regions showed significant variation. Phylogenetic analysis confirmed that CKX and XG is not closely related to  HZP. Additionally, 56 pairs of primers were designed based on polymorphic sites, with 17 primers successfully validated for assessing polymorphic sites, providing essential tools for the identification and genetic study of tea-oil Camellia species.

According to the study's lead researcher, Daojun Zheng, “The present study provided high-quality chloroplast genomes and reliable molecular marker resources for future tea-oil Camellia research.”

In summary, this study primarily investigated the chloroplast genomes of C. meiocarpa and C. oleifera, revealing significant genetic differences that support C. meiocarpa as an independent species. The development of 17 primers enhances species identification and resource assessment, benefiting breeding programs and production practices. These findings provide high-quality chloroplast genomes and reliable molecular marker resources for future tea-oil Camellia research.

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References

DOI

10.48130/tp-0024-0022

Original Source URL

https://doi.org/10.48130/tp-0024-0022

Funding information

This study was supported by the Project of Sanya Yazhou Bay Science and Technology City (Grant No. SCKJ-JYRC-202258), Southern Breeding Project of Sanya National Southen Breeding Research Academy of Chinese Academy of Agricultural Sciences (Grant No. YYLH10), the National Natural Science Foundation of China (31860082), Hainan Province Science and Technology Special Fund (FW20230002), Scientific and technological innovation team of Hainan Academy of Agricultural Sciences (HAAS2023TDYD05), introduce talents to initiate scientific research projects of Hainan Academy of Agricultural Sciences (HAAS2023RCQD13).

About Tropical Plants

Tropical Plants (e-ISSN 2833-9851) is the official journal of Hainan University and published by Maximum Academic Press. Tropical Plants undergoes rigorous peer review and is published in open-access format to enable swift dissemination of research findings, facilitate exchange of academic knowledge and encourage academic discourse on innovative technologies and issues emerging in tropical plant research.