Pesticide exposure linked to stillbirth risk in new study

Living less than about one-third of a mile from pesticide use prior to conception and during early pregnancy could increase the risk of stillbirths, according to new research led by researchers at the Mel and Enid Zuckerman College of Public Health and Southwest Environmental Health Sciences Center.

Researchers found that during a 90-day pre-conception window and the first trimester of pregnancy, select pesticides, including organophosphates as a class, were associated with stillbirth.

The paper, “Pre-Conception And First Trimester Exposure To Pesticides And Associations With Stillbirth,” was published in the American Journal of Epidemiology.

“In this study, some specific ingredients stood out due to their significant associations with stillbirth risk,” said first author Melissa Furlong, PhD, who studies the chronic health effects of environmental contaminants as an assistant professor and environmental epidemiologist at the Zuckerman College of Public Health and a member of the Southwest Environmental Health Sciences Center at the R. Ken Coit College of Pharmacy. “These findings underscore the importance of considering individual pesticides rather than just the overall pesticide class, as specific chemical compounds may pose unique risks. It also highlights the potential for pre-pregnancy exposures to affect reproductive outcomes.”

To conduct the study, researchers linked Arizona pesticide use records for 27 different pesticides with state birth certificate data that included 1,237,750 births and 2,290 stillbirths from 2006 to 2020.

They found that living within .31 miles (500 meters) of specific pyrethroid, organophosphate or carbamate pesticide applications during a 90-day pre-conception window or the first trimester was associated with an increased risk of stillbirth. 

Specifically, the pesticides cyfluthrin, zeta-cypermethrin, organophosphates as a class, malathion, carbaryl and propamocarb hydrochloride were linked to increased stillborn births pre-conception. During the first trimester, fenpropathrin, permethrin, organophosphates as a class, acephate and formetanate hydrochloride were associated with stillbirths.

“Among organophosphates, acephate showed the strongest effect estimates on stillbirth, so that exposure to acephate in the first trimester was associated with a doubling of risk,” said co-author Paloma Beamer, PhD, a professor and interim associate dean at the Zuckerman College of Public Health and a member of the Southwest Environmental Health Sciences Center, U of A Health Sciences Asthma and Airway Disease Research Center and BIO5 Institute.  “Within the pyrethroid class, cyfluthrin exposure during the 90 days prior to conception almost doubled the risk of stillbirth.”

Pesticides are chemical substances used to control pests in various settings. They are commonly categorized into different classes, such as organophosphates, pyrethroids and carbamates. The primary route of exposure for most people is through diet, but household use, agricultural drift and occupational exposure are also significant pathways.

Researchers say while some pesticides may not have been directly implicated in this study, they could still pose risks to maternal and fetal health.

Pregnant women may be particularly vulnerable to the adverse effects of pesticide exposure due to physiological changes during pregnancy, such as increased metabolic rate, altered hormone levels and changes in the immune system. The developing fetus may be more susceptible to the toxic effects of pesticides during this period of rapid growth and development.

“Further research is essential to fully understand the safety profiles of various pesticides and to understand the underlying mechanisms of pesticide-induced stillbirth,” Furlong said. “This study underscores the need to develop strategies for mitigating exposure to protect maternal and fetal health.”

Co-authors from the University of Arizona include Alfred Fournier, PhD, an associate specialist in entomology in the College of Agriculture, Life and Environmental Sciences; Peter Ellsworth, PhD, professor in the Department of Entomology at the College of Agriculture, Life and Environmental Sciences; Avelino Arrelano, PhD, an associate professor of data assimilation and atmospheric chemistry at the College of Science Department of Hydrology and Atmospheric Sciences; and Edward Bedrick, PhD, a professor in the Department of Epidemiology and Biostatistics at the Zuckerman College of Public Health. Other co-authors include Kimberly Parra, PhD, who was a doctoral candidate at the Zuckerman College of Public Health when the research was conducted and is now a research fellow at Harvard University’s T.H. Chan School of Public Health; Beate Ritz, MD, PhD, and Kimberly Paul, PhD, from the University of California, Los Angeles; and Myles Cockburn, PhD, from University of Southern California.

This research was supported in part by the National Institute of Environmental Health Sciences, a division of the National Institutes of Health, under Award Nos. R00ES028743 and P30ES006694.Living less than about one-third of a mile from pesticide use prior to conception and during early pregnancy could increase the risk of stillbirths, according to new research led by researchers at the Mel and Enid Zuckerman College of Public Health and Southwest Environmental Health Sciences Center.

Researchers found that during a 90-day pre-conception window and the first trimester of pregnancy, select pesticides, including organophosphates as a class, were associated with stillbirth.

The paper, “Pre-Conception And First Trimester Exposure To Pesticides And Associations With Stillbirth,” was published in the American Journal of Epidemiology.

“In this study, some specific ingredients stood out due to their significant associations with stillbirth risk,” said first author Melissa Furlong, PhD, who studies the chronic health effects of environmental contaminants as an assistant professor and environmental epidemiologist at the Zuckerman College of Public Health and a member of the Southwest Environmental Health Sciences Center at the R. Ken Coit College of Pharmacy. “These findings underscore the importance of considering individual pesticides rather than just the overall pesticide class, as specific chemical compounds may pose unique risks. It also highlights the potential for pre-pregnancy exposures to affect reproductive outcomes.”

To conduct the study, researchers linked Arizona pesticide use records for 27 different pesticides with state birth certificate data that included 1,237,750 births and 2,290 stillbirths from 2006 to 2020.

They found that living within .31 miles (500 meters) of specific pyrethroid, organophosphate or carbamate pesticide applications during a 90-day pre-conception window or the first trimester was associated with an increased risk of stillbirth. 

Specifically, the pesticides cyfluthrin, zeta-cypermethrin, organophosphates as a class, malathion, carbaryl and propamocarb hydrochloride were linked to increased stillborn births pre-conception. During the first trimester, fenpropathrin, permethrin, organophosphates as a class, acephate and formetanate hydrochloride were associated with stillbirths.

“Among organophosphates, acephate showed the strongest effect estimates on stillbirth, so that exposure to acephate in the first trimester was associated with a doubling of risk,” said co-author Paloma Beamer, PhD, a professor and interim associate dean at the Zuckerman College of Public Health and a member of the Southwest Environmental Health Sciences Center, U of A Health Sciences Asthma and Airway Disease Research Center and BIO5 Institute.  “Within the pyrethroid class, cyfluthrin exposure during the 90 days prior to conception almost doubled the risk of stillbirth.”

Pesticides are chemical substances used to control pests in various settings. They are commonly categorized into different classes, such as organophosphates, pyrethroids and carbamates. The primary route of exposure for most people is through diet, but household use, agricultural drift and occupational exposure are also significant pathways.

Researchers say while some pesticides may not have been directly implicated in this study, they could still pose risks to maternal and fetal health.

Pregnant women may be particularly vulnerable to the adverse effects of pesticide exposure due to physiological changes during pregnancy, such as increased metabolic rate, altered hormone levels and changes in the immune system. The developing fetus may be more susceptible to the toxic effects of pesticides during this period of rapid growth and development.

“Further research is essential to fully understand the safety profiles of various pesticides and to understand the underlying mechanisms of pesticide-induced stillbirth,” Furlong said. “This study underscores the need to develop strategies for mitigating exposure to protect maternal and fetal health.”

Co-authors from the University of Arizona include Alfred Fournier, PhD, an associate specialist in entomology in the College of Agriculture, Life and Environmental Sciences; Peter Ellsworth, PhD, professor in the Department of Entomology at the College of Agriculture, Life and Environmental Sciences; Avelino Arrelano, PhD, an associate professor of data assimilation and atmospheric chemistry at the College of Science Department of Hydrology and Atmospheric Sciences; and Edward Bedrick, PhD, a professor in the Department of Epidemiology and Biostatistics at the Zuckerman College of Public Health. Other co-authors include Kimberly Parra, PhD, who was a doctoral candidate at the Zuckerman College of Public Health when the research was conducted and is now a research fellow at Harvard University’s T.H. Chan School of Public Health; Beate Ritz, MD, PhD, and Kimberly Paul, PhD, from the University of California, Los Angeles; and Myles Cockburn, PhD, from University of Southern California.

This research was supported in part by the National Institute of Environmental Health Sciences, a division of the National Institutes of Health, under Award Nos. R00ES028743 and P30ES006694.