Green synthesis of copper oxide nanoparticles from mangifera indica: A sustainable solution for agricultural disease management

A research team has successfully synthesized green copper oxide nanoparticles (CuO.NPs) from Mangifera indica (M. indica) leaf extract. The CuO.NPs showed potent activity against gram-positive and negative bacteria, as well as fungicidal effects on persimmon fruit pathogens. This advancement holds significant value for agriculture, offering a biocompatible and eco-friendly method to combat phytopathogenic diseases. Future applications of this technology could revolutionize disease management in crops, potentially enhancing agricultural productivity and sustainability.

A sustainable increase in agricultural production is needed due to a predicted doubling in global crop demand from 2005 to 2050. However, the rapid propagation of plant pathogens threatens the sustainability of the food supply. Crop production relies heavily on synthetic agrochemicals to combat plant diseases, but this approach contributes to environmental harm and pathogen resistance. These issues underscore the need for innovative, eco-friendly disease control methods. There has been a lot of research done recently on the green synthesis of various antimicrobial nanoparticles. Copper, a high-performance metal used in agriculture to combat pathogenic attacks, has received less attention.

A study (DOI: 10.48130/frures-0024-0015) published in Fruit Research on 03 June 2024, aims to synthesize eco-friendly and economically viable M. indica-mediated CuO.NPs to develop sustainable, biocompatible solutions for phytopathogen management.

Firstly, M. indica leaf extract was employed to synthesize copper oxide nanoparticles (CuO.NPs), and the synthesis was confirmed by a color change from yellow to copper-reddish. Then, the spectral and morphological characterization biosynthesized were observed using FTIR, XRD, and TEM. The FT-IR analysis verified the existence of functional groups such as C-N and O-H in the green synthesized CuO.NPs, which possess the potential to effectively inhibit pathogenic growth. XRD was carried out to demonstrate the crystalline nature and size of nanoparticles using the Scherrer formula. The TEM analysis indicated that the CuO.NPs display a spherical morphology and are effectively dispersed without aggregating, with sizes ranging from 40−80 nm. Further, the antibacterial activity of CuO.NPs were evaluated at different doses (30, 60, and 100 μg·mL⁻¹) both in vitro and in vivo. The CuO.NPs exhibited significant antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with higher doses showing greater inhibition zones. The antifungal activity in vivo was tested on Diospyros kaki (persimmon fruit), where CuO.NPs significantly reduced disease incidence. Additionally, the nanoparticles also demonstrated strong antioxidant activity, comparable to ascorbic acid. These results suggest that green synthesized CuO.NPs have substantial potential for use as eco-friendly, cost-effective antimicrobial agents in agriculture.

According to the study's lead researcher, Cheng Song, “This work offers a new avenue for additional investigations of metallic nanoparticles in vital domains like phytopathology and agriculture. The use of this environmentally friendly method of biogenic nanoparticles may help to reduce the fungal infection in sweet fruit and improve the socio-economic standing of farmers.”

In summary, this research showcases the green synthesis of CuO.NPs using M. indica leaf extract. Characterized by FTIR, XRD, and TEM, the CuO.NPs demonstrated significant antimicrobial activity against E. coli and S. aureus, fungicidal effects on R. oryzae, and strong antioxidant properties. These findings highlight the potential of eco-friendly CuO.NPs as cost-effective antimicrobial agents in agriculture. Future research should focus on optimizing synthesis, expanding applications, and scaling up production to enhance sustainable crop protection.

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References

DOI

10.48130/frures-0024-0015

Original Source URL

https://doi.org/10.48130/frures-0024-0015

Funding information

This work was supported by the Key R&D Project of the 14^th Five Year Plan of China (2023YFC3503804), Startup fund for high-level talents of West Anhui University (WGKQ2022025), the Open Fund of Anhui Engineering Research Center for Eco-agriculture of Traditional Chinese Medicine (WXZR202318), and Demonstration Experiment Training Center of Anhui Provincial Department of Education (2022sysx033). The authors extend their appreciation to the Deanship of Scientific Research at King Khalid University, Saudi Arabia for funding this work through Small Groups Project under Grant Number (RGP.2/176/44).

About Fruit Research 

Fruit Research (e-ISSN 2769-4615) is an open access, online-only journal to publish original research articles, new methods, perspectives, and reviews on novel discoveries in all areas of fruit science. The journal will focus on publishing basic and translational research with a broad impact in fruit science.