Nature's gender reveal: Key gene controls female flowers in Cucurbita pepo

A transformative study has pinpointed the CpMYB62 transcription factor as a key determinant in the sex differentiation of Cucurbita pepo flowers. This gene mutation, which impedes the female floral transition, may be partially reversed by ethylene, indicating its critical role in ethylene-mediated sex determination pathways. The implications of this discovery could extend to advancing horticultural practices and enhancing crop yields.

Sex determination in cucurbits like squash is influenced by a complex interplay of environmental, hormonal, and genetic factors, with ethylene being the primary hormone promoting female flower development. Despite understanding ethylene's role, the upstream regulators controlling its production remained elusive. The conversion of female flowers to male flowers in certain mutants highlighted gaps in knowledge regarding genetic influences. Based on these challenges, an in-depth study was essential to uncover the genetic mechanisms regulating sex determination and improve crop yields through targeted genetic manipulation.

Researchers from the University of Almería published a new study in Horticulture Research on April 22, 2024. The study (DOI: 10.1093/hr/uhae115) focuses on the transcription factor CpMYB62 and its essential role in female flower development in Cucurbita pepo. Through the identification and characterization of the myb62 mutation, the researchers discovered that CpMYB62 regulates ethylene production, which is crucial for female flower formation. This study provides valuable insights into the genetic mechanisms governing sex determination in squash, offering potential strategies for enhancing crop yields.

The study identified the myb62 mutation in ethylmethane sulfonate (EMS) mutant lines, which converted all female flowers into male flowers. Using Bulk Segregant Analysis Sequencing (BSA-seq) and Whole Genome Sequencing (WGS), researchers discovered that the myb62 mutation is a C>T transition in the CpMYB62 gene, causing a premature stop codon and a truncated transcription factor. This mutation leads to reduced ethylene production, crucial for female flower development. Exogenous ethylene application partially rescued the female flowering phenotype, confirming CpMYB62's role in ethylene regulation. Transcriptomic analysis revealed that CpMYB62 regulates key sex-determining genes, upregulating those involved in carpel development (CpACO2B, CpACS27A) and downregulating those arresting carpel development (CpWIP1). CpMYB62 thus occupies a pivotal position in the gene network controlling sex determination in Cucurbita pepo, promoting female flower development by activating ethylene biosynthesis and other sex-determining genes.

Dr. Cecilia Martínez, one of the lead researchers, stated, "This discovery sheds light on the genetic mechanisms controlling female flower development in squash. Understanding the role of CpMYB62 in regulating ethylene production opens new avenues for improving crop yield by manipulating flower development genetically."

The findings of this study have significant implications for agricultural practices. By manipulating the CpMYB62 gene, it may be possible to enhance female flower production, thereby increasing fruit yield in squash and other cucurbits. This research not only advances our understanding of plant genetics but also offers practical solutions for improving crop productivity in the horticulture industry.

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References

DOI

10.1093/hr/uhae115

Original Source URL

https://doi.org/10.1093/hr/uhae115

Funding information

This work was supported by grant PID2020-118080RB-C21, funded by the Spanish Ministry of Science and Innovation together with EU FEDER funds. M.S. acknowledges the D.I scholarship program from MCI with the company Green Breeding Biotech SL, J.I.-M. acknowledges the FPI scholarship program from MEC, and A.G. received a Margarita Salas postdoctoral fellowship.

About Horticulture Research

Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.