Pineapple peel's red secret: Scientists uncover the molecular mechanism behind its color

A pivotal study has identified AcMYB266 as a crucial transcription factor that governs the red coloration in pineapple peels, a genetically complex trait. This discovery is set to revolutionize pineapple breeding, offering a pathway to enhance fruit quality and appearance by manipulating anthocyanin levels, thereby unlocking both commercial and nutritional potentials.

Pineapple peel coloration, especially the red hue, significantly enhances its commercial and aesthetic value. However, the genetic mechanisms driving this trait are not well understood, complicating breeding efforts for red-skinned varieties. Previous studies have identified various genes involved in anthocyanin synthesis, but their precise regulatory roles remain unclear. Due to these challenges, an in-depth investigation into the genetic factors influencing pineapple peel coloration is essential to develop high-quality varieties that meet market demands.

Researchers from the South China Agricultural University, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, and Guangdong Eco-Engineering Polytechnic published their findings (DOI: 0.1093/hr/uhae116) on April 25, 2024, in Horticulture Research. The study investigates the role of AcMYB266, a transcription factor, in regulating the red coloration of pineapple peel by promoting anthocyanin synthesis. This discovery provides new insights into the genetic mechanisms behind fruit peel pigmentation, with significant implications for breeding red-skinned pineapple varieties.

The research team identified AcMYB266 as a key transcription factor specifically expressed in the pineapple peel, regulating anthocyanin accumulation. Through transgenic experiments in pineapple, Arabidopsis, and tobacco, they demonstrated that overexpression of AcMYB266 leads to increased anthocyanin levels, resulting in red pigmentation. The study also found that variations in the promoter sequences of AcMYB266 contribute to the different red coloration patterns observed in various pineapple varieties. Notably, AcMYB266 is part of a unique gene cluster in Ananas species, which includes other MYB genes that regulate anthocyanin synthesis in different tissues, such as leaves and petals. This gene cluster represents an interesting case of subfunctionalization after gene duplication, where each gene has evolved to regulate anthocyanin accumulation in specific tissues. These findings offer valuable insights for breeding high-quality red-skinned pineapple varieties.

Dr. Chengjie Chen, one of the leading researchers, stated, "Our findings on AcMYB266 provide valuable insights into the genetic regulation of anthocyanin accumulation in pineapple. This not only enhances our understanding of fruit coloration but also opens new avenues for breeding programs aimed at producing high-quality red-skinned pineapple varieties."

The identification of AcMYB266 and its role in anthocyanin accumulation holds significant implications for pineapple breeding. By manipulating this gene, it is possible to develop new pineapple varieties with enhanced aesthetic and nutritional value, meeting both market demand and consumer preferences. This study also serves as a model for exploring gene subfunctionalization in other plant species, potentially leading to broader agricultural advancements.

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References

DOI

10.1093/hr/uhae116

Original Source URL

https://doi.org/10.1093/hr/uhae116

Funding information

Anthocyanin accumulation types in fruit peel of representative pineapple varieties and the corresponding AcMYB266 promoter activation ability.

About Horticulture Research

Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.