Pear-derived discovery: A genetic mechanism to fortify crops against drought

A pivotal study has shed light on a critical genetic mechanism that boosts plants' ability to withstand drought. The research uncovers the role of the transcription factor PbERF3, native to wild pears, which works in concert with the protein PbHsfC1a to regulate genes key to drought tolerance. This breakthrough could significantly inform the cultivation of crops with superior resilience to water shortages, offering a vital solution to the pressing environmental demands of our time.

Drought significantly impacts agricultural productivity and plant survival. Traditional methods for improving drought resistance, such as selective breeding and genetic modification, have had limited success. Therefore, identifying genetic components that enhance drought tolerance is crucial. Research has shown that understanding and manipulating these genetic factors can lead to the development of more resilient crops. Due to these challenges, there is a pressing need to delve deeper into the genetic mechanisms of drought resistance to develop effective solutions for sustainable agriculture.

A team of scientists from the College of Horticulture at Nanjing Agricultural University has published a study (DOI: 10.1093/hr/uhae090) on March 30, 2024, in Horticulture Research. The research focuses on the transcription factor PbERF3 from wild pear, demonstrating its role in enhancing drought resistance by interacting with another protein, PbHsfC1a. This interaction regulates the expression of genes involved in hydrogen peroxide transport and abscisic acid biosynthesis, critical for drought tolerance.

The study demonstrates that overexpressing PbERF3 in pear callus and Arabidopsis enhances drought resistance by restoring redox balance and activating key drought stress pathways. PbERF3 interacts with PbHsfC1a, forming a heterodimer that binds to the promoters of PbPIP1;4 and PbNCED4, which are essential for hydrogen peroxide transport and abscisic acid biosynthesis. This interaction activates critical signaling pathways that improve drought tolerance. Silencing PbERF3 resulted in reduced drought resistance, underscoring its vital role in stress response. Additionally, the research shows that PbERF3 directly stimulates the transcription of PbPIP1;4, enhancing the plant's ability to manage oxidative stress. These findings reveal a novel regulatory module that plants use to combat drought stress, providing insights into developing genetically modified crops with improved drought resistance.

Dr. Xiaosan Huang, the corresponding author, stated, "Our findings reveal a critical regulatory network that wild pears use to combat drought stress. Understanding this mechanism opens up new possibilities for engineering drought-resistant crops, which is vital in the face of increasing climate variability."

This discovery provides a foundation for developing genetically modified crops with enhanced drought tolerance, potentially improving agricultural resilience. By leveraging the PbERF3-PbHsfC1a regulatory module, scientists can create plants better equipped to withstand drought conditions, ensuring food security and sustainable agricultural practices in arid regions.

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References

DOI

10.1093/hr/uhae090

Original Source URL

https://doi.org/10.1093/hr/uhae090

Funding information

This work has been supported by the National Key Research and Development Program of China (2019YFD1000102), the Key Research and Development Program of Jiangsu Province (BE2023328), the National Science Foundation of China (32072538), the Jiangsu Agriculture Science and Technology Innovation Fund (CX(22)3046), and Postgraduate Research and Practice Innovation Program of Jiangsu (KYCX23_0795).

About Horticulture Research

Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.