image: The beneficial characteristics of Nbs for food allergen surveillance. (A) Almost 90% of all serious food allergies are related to proteins (allergens) in nine foods including egg, milk, peanut, and tree nuts. The incidence of food allergen exposure occures during the complete food chain from manufacturing to transportation with unexpected contamination or intentional supplementation; (B) The comprehensive analysis of food allergen in the complete food chain is proposed with Nb-based immunoassay, and certain properties of Nbs potentially benefit food allergen surveillance with Nb-based immunoassays; and (C) The unique characteristics facilitate Nbs as the perfect candidates for research and application in the field of food allergen surveillance.
Credit: Food Quality and Safety
Recent advancements show nanobodies from camelid antibodies excel in food allergen detection with superior stability, specificity, and cost-effectiveness. This innovative approach aims to improve accuracy and efficiency, crucial for preventing severe allergic reactions. The study highlights nanobodies' potential in reliable immunoassays, addressing rising food allergies and enhancing safety measures.
Food allergies pose significant health risks, affecting millions worldwide, with the prevalence rising over the past decades. Traditional detection methods, such as monoclonal and polyclonal antibodies, are often costly, labor-intensive, and prone to cross-reactions. The need for accurate, efficient, and cost-effective allergen detection methods is more pressing than ever. Due to these challenges, there is a growing interest in exploring new detection strategies. Based on these challenges, it is essential to conduct in-depth research to develop advanced detection techniques that can better safeguard public health.
Researchers from Tianjin Key Laboratory of Food Science and Health, Nankai University, China have made significant strides in this field. Their review (DOI: 10.1093/fqsafe/fyae018), published on March 28, 2024, in the journal Food Quality and Safety, introduces the use of nanobodies for food allergen surveillance, potentially revolutionizing the way we detect and manage food allergies.
The study highlights the advantages of nanobodies over traditional antibodies in food allergen detection. Derived from camelid heavy-chain antibodies, nanobodies are small, stable, and highly specific, making them ideal for robust immunoassays. They minimize cross-reactions and effectively detect allergens like macadamia, peanut, lupin, and milk proteins. A key highlight is their ability to target conformational epitopes, offering higher precision in allergen detection, especially in complex food matrices. The research also demonstrates the versatility and efficiency of nanobodies in various detection methods, such as Enzyme-Linked Immunosorbent Assay (ELISA) and electrochemical immunoassays, showcasing their broad applicability.
Prof. Dr. Shuo Wang, one of the leading researchers in this field and the leader of this research, stated, "The development of nanobody-based immunoassays marks a significant advancement in food allergen detection. Nanobodies offer unparalleled stability and specificity, addressing the critical need for more accurate and efficient methods to protect sensitive individuals from food allergens. This research paves the way for future innovations in food safety."
The research implications are significant. Nanobody-based detection methods could revolutionize food allergen surveillance, offering reliable, cost-effective solutions, enhancing food safety, and reducing allergic risks. Additionally, this study opens new avenues for developing therapeutic strategies and diagnostic tools, highlighting nanobodies' versatility in biomedical applications.
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References
DOI
Original Source URL
https://doi.org/10.1093/fqsafe/fyae018
Funding information
This research was funded by the National Natural Science Foundation of China (No. 32272403) and the National Key Research and Development Program of China (No. 2022YFF1102400).
About Food Quality and Safety
Food Quality and Safety (FQS) is an open access, international, peer-reviewed journal providing a platform to highlight emerging and innovative science and technology in the agro-food field, publishing up-to-date research in the areas of food quality, food safety, food nutrition and human health. It is covered by SCI-E and the 2022 Impact Factor (IF)=5.6, 5-yr IF=6.2.
Journal
Food Quality and Safety
Subject of Research
Not applicable
Article Title
Nanobody-based food allergen surveillance: current status and prospects
Article Publication Date
28-Mar-2024
COI Statement
The authors declare that they have no competing interests.