Unlocking banana disease resistance: key enzymes identified for phytoalexin synthesis

A breakthrough in banana disease resistance was achieved with the identification of novel O-methyltransferases (OMTs) involved in the biosynthesis of phenylphenalenone phytoalexins. These compounds, found in wild bananas, hold promise for developing disease-resistant commercial cultivars. Researchers assembled a near-complete genome of Musella lasiocarpa and identified three novel OMTs that enhance the antifungal properties of these phytoalexins, providing a genetic resource for improving banana disease resistance through molecular breeding.

Bananas are a vital crop globally, but their yields are threatened by various diseases, particularly banana fusarium wilt caused by Fusarium oxysporum. Traditional control methods, including chemical pesticides, pose environmental risks and are not always effective. Wild banana relatives, which possess greater genetic diversity and higher concentrations of defense-related compounds, offer potential solutions. Based on these challenges, further research into the genetic mechanisms behind banana disease resistance is essential.

Researchers from the Jiangsu Key Laboratory for the Research and Utilization of Plant Resources and collaborators published a study (DOI:10.1093/hr/uhae042) on March 8, 2024, in Horticulture Research. They assembled a nearly gapless genome of Musella lasiocarpa, a wild banana relative, to investigate the biosynthesis of phenylphenalenone (PhPN) phytoalexins. This study identified three novel O-methyltransferases (OMTs) involved in enhancing the antifungal properties of these compounds, paving the way for developing disease-resistant banana varieties.

The study focused on the biosynthesis pathway of PhPN phytoalexins, which are natural defense compounds in bananas. Using advanced genomic techniques, the team assembled a high-quality, nearly complete genome of Musella lasiocarpa. They integrated transcriptomic and metabolomic data to identify candidate genes involved in PhPN biosynthesis. Through phylogenetic analysis and in vitro enzymatic assays, three novel OMTs were characterized. These enzymes, Ml01G0494, Ml04G2958, and Ml08G0855, showed significant roles in the methylation modification of PhPNs, enhancing their antifungal activity against Fusarium oxysporum. Ml08G0855, in particular, was found to be a multifunctional enzyme targeting multiple hydroxyl groups in PhPN structures. The study also revealed that the methylation of PhPNs significantly boosts their antifungal properties, providing a potential genetic resource for improving disease resistance in bananas through molecular breeding.

Dr. Yu Chen, one of the corresponding authors, stated, "This research provides crucial insights into the genetic basis of disease resistance in bananas. By understanding and leveraging the biosynthetic pathways of phenylphenalenone phytoalexins, we can develop more resilient banana cultivars, ensuring better crop yields and sustainability in the face of increasing agricultural challenges."

The application of this research is twofold: it offers a direct application in banana breeding programs to enhance disease resistance through molecular techniques and provides a foundation for further studies into the role of phytoalexins in plant-pathogen interactions. The implications are far-reaching, potentially leading to the cultivation of hardier banana varieties that can withstand climate change-induced stress and reduce the environmental impact of disease management practices.

###

References

DOI

10.1093/hr/uhae042

Original Source URL

https://doi.org/10.1093/hr/uhae042

Funding information

This work was supported by the National Natural Science Foundation of China (grants 32070360, 32200326, and 22207047), the Natural Science Foundation of Jiangsu Province (BK20220752 and BK20220749), and the Jiangsu Institute of Botany Talent Fund (JIBTF202304). We thank Professor Zefu Wang (Nanjing Forestry University) for critical reading of the manuscript and Emily Wheeler for editorial assistance.

About Horticulture Research

Horticulture Research is an open access journal of Nanjing Agricultural University and ranked number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. The journal is committed to publishing original research articles, reviews, perspectives, comments, correspondence articles and letters to the editor related to all major horticultural plants and disciplines, including biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.